Recombinant Rabbit Anti-CHRM2 Antibody (clone R01-9B9) (CAT#: VS3-FY383)
This product is a recombinant rabbit antibody that recognizes human and rat CHRM2 protein. It can detect a band of approximately 52 kDa (observedd molecular weight: 52 kDa).
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WB
Figure 1. Recombinant Rabbit Anti-CHRM2 Antibody (clone R01-9B9) in WB.
Western blot analysis of Muscarinic acetylcholine receptor 2/CM2 in K562, C6, Hela lysates using Muscarinic acetylcholine receptor M2 Antibody.
Specifications
- Immunogen
- A synthetic peptide of human Muscarinic Acetylcholine Receptor 2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CHRM2
- Species Reactivity
- Human, Rat
- Clone
- R01-9B9
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 52 kDa; Observed MW: 52 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cholinergic Receptor Muscarinic 2; Acetylcholine Receptor, Muscarinic 2; Muscarinic Acetylcholine Receptor M2; Cholinergic Receptor, Muscarinic 2; Muscarinic M2 Receptor; 7TM Receptor; HM2
- Gene ID
- 1129
- UniProt ID
- P08172
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
- Cellular Localization
- Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse
- Post Translation Modifications
- Phosphorylated in response to agonist treatment.
- Protein Refseq
- NP_000730.1; NP_001006627.1; NP_001006628.1
- Function
- The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-205 | Recombinant Anti-human CHRM2 Antibody | ELISA, WB, IP, FuncS | IgG |
| MHH-205 | Recombinant Human Anti-human CHRM2 Antibody | ELISA, WB, FC, FuncS | IgG |
| VS3-QX236 | Mouse Anti-CHRM2 Recombinant Antibody (clone 1424CT461.78.60) | ICC, IHC, FC | Mouse IgG1, κ |
| VS3-CJ487 | Mouse Anti-CHRM2 Recombinant Antibody (VS3-CJ487) | WB, IF, IHC-P, FC | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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