Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) (CAT#: ZG-0177J)
This product is a mouse antibody that recognizes COL3A1.
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WB
Figure 1 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in WB
Western blot analysis of lysates from 1) MCF7 , 2) Hela, 3) 293T cells, :reen:primary Antibody was diluted at 1:1000, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C 1 hour. :ed:Actin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°C over night, secondary Antibody was diluted at 1:10000, 37°C 1 hour.
IHC
Figure 2 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Collagen III Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 3 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.Collagen III Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IF
Figure 4 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in IF
Immunofluorescence analysis of Mouse-liver tissue. 1.Collagen III Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
WB
Figure 5 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in WB
Western blot analysis of Hela, diluted at 1:1000.
Specifications
- Immunogen
- Synthetic Peptide of Collagen III
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human COL3A1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- Q76
- Applications
- WB, IHC-P, IF/ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence/Immunocytochemistry.
Target
- Alternative Names
- EDS4A; EDSVASC; PMGEDSV; collagen alpha-1(III) chain; Ehlers-Danlos syndrome type IV, autosomal dominant; alpha-1 type III collagen; alpha1 (III) collagen; collagen, fetal; collagen, type III, alpha 1
- Gene ID
- 1281
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0910-FY-F(E) | Mouse Anti-COL3A1 Recombinant Antibody (clone 4E5F4A11); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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