Mouse Anti-COL3A1 Recombinant Antibody (clone Q76)
CAT#: ZG-0177J
This product is a mouse antibody that recognizes COL3A1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in WB
Western blot analysis of lysates from 1) MCF7 , 2) Hela, 3) 293T cells, :reen:primary Antibody was diluted at 1:1000, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C 1 hour. :ed:Actin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°C over night, secondary Antibody was diluted at 1:10000, 37°C 1 hour.
IHC
Figure 2 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Collagen III Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 3 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.Collagen III Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IF
Figure 4 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in IF
Immunofluorescence analysis of Mouse-liver tissue. 1.Collagen III Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
WB
Figure 5 Mouse Anti-COL3A1 Recombinant Antibody (clone Q76) in WB
Western blot analysis of Hela, diluted at 1:1000.
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7583/1801_B1_14_cr59c25470e819f_selected.jpg
Normal Tissue
Figure 2 IHC staining of human nasopharynx
Immunohistochemical staining of human nasopharynx shows distinct positivity in fibroblasts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7583/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human bronchus
Immunohistochemical staining of human bronchus shows distinct positivity in the submucosa.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16766/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic and membranous positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_A_9_3.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59993/130279_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, ASC diff, ASC TERT1, fHDF/TERT166, HHSteC, HSkMC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168542-COL3A1
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Specifications
- Immunogen
- Synthetic Peptide of Collagen III
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human COL3A1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- Q76
- Applications
- WB, IHC-P, IF, ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence/Immunocytochemistry.
Target
- Alternative Names
- EDS4A; EDSVASC; PMGEDSV; collagen alpha-1(III) chain; Ehlers-Danlos syndrome type IV, autosomal dominant; alpha-1 type III collagen; alpha1 (III) collagen; collagen, fetal; collagen, type III, alpha 1
- Gene ID
- 1281
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| HPAB-0910-FY-F(E) | Mouse Anti-COL3A1 Recombinant Antibody (clone 4E5F4A11); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0910-FY-S(P) | Mouse Anti-COL3A1 Recombinant Antibody (clone 4E5F4A11); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0640F | Rabbit Anti-COL3A1 Recombinant Antibody (ZG-0640F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
| ZG-0642F | Rabbit Anti-COL3A1 Recombinant Antibody (ZG-0642F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
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| VS7-HM759 | Mouse Anti-COL3A1 Recombinant Antibody (clone 8C1G11) | IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY502 | Anti-COL3A1 Immunohistochemistry Kit | IHC | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC68 | Recombinant Anti-COL3A1 Eliminating Antibody, pH-Sensitive (VS-0625-YC68) | Antigen-Sweeping In Vivo. | Mouse IgG |
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