CytoStream™ Rabbit Anti-COL4A1 Polyclonal Antibody (VS13-YC236) (CAT#: VS13-YC236)
The CytoStream™ Rabbit Anti-COL4A1 Polyclonal Antibody is specific for human, mouse and rat COL4A1. COL4A1 encodes a collagen chain influencing extracellular matrix structures, linked to disorders like retinal artery and schizencephaly through integrin pathways. The anti-COL4A1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1-2 μg per 1x 10⁶ cells).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows positivity in basement membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows positivity in basement membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/5012_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/5012_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_9_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_8_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_8_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_7_5.jpg
Figure 6 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_4_6.jpg
Figure 7 Placenta
(Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_3_7.jpg
(Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_3_7.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, EFO-21, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187498-COL4A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187498-COL4A1
Specifications
- Immunogen
- Recombinant human Collagen alpha-1(IV) chain, aa 1571-1669, conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat COL4A1
- Species Reactivity
- Human, Mouse, Rat, Dog (Predicted), Pig (Predicted), Cow (Predicted), Rabbit (Predicted), Chicken (Predicted), Horse (Predicted)
- Applications
- ELISA, IHC-P, IHC-Fr, FC, IF
- Conjugate
- NONE
- Related Disease
- Retinal Arteries, Tortuosity Of and Schizencephaly
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1-2 μg per 1x 10⁶ cells
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- Collagen Type IV Alpha 1 Chain; Collagen Of Basement Membrane, Alpha-1 Chain; Collagen IV, Alpha-1 Polypeptide; Collagen, Type IV, Alpha 1; Collagen Alpha-1(IV) Chain; COL4A1 NC1 Domain;
- Gene ID
- 1282
- UniProt ID
- P02462
- Long Name
- Collagen Type IV Alpha 1 Chain
- Cellular Localization
- Secreted; Extracellular space; Extracellular matrix; Basement membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0561F | Mouse Anti-COL4A1 Recombinant Antibody (clone 24D1) | IHC-P, IF, ELISA, RIA, WB | Mouse IgG1 |
| ZG-0576F | Mouse Anti-COL4A1 Recombinant Antibody (clone COL 94) | IHC-Fr, IHC-P, WB | Mouse IgG1 |
| ZG-0645F | Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0645F) | IHC-P, IF, ELISA, RIA | Rabbit IgG |
| ZG-0646F | Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0646F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
| ZG-0647F | Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0647F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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