CytoStream™ Rabbit Anti-COL4A1 Polyclonal Antibody (VS13-YC236) (CAT#: VS13-YC236)
The CytoStream™ Rabbit Anti-COL4A1 Polyclonal Antibody is specific for human, mouse and rat COL4A1. COL4A1 encodes a collagen chain influencing extracellular matrix structures, linked to disorders like retinal artery and schizencephaly through integrin pathways. The anti-COL4A1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1-2 μg per 1x 10⁶ cells).
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows positivity in basement membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows positivity in basement membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/5012_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/5012_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_9_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_8_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_8_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_7_5.jpg
Figure 6 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_4_6.jpg
Figure 7 Placenta
(Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_3_7.jpg
(Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_3_7.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, EFO-21, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187498-COL4A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187498-COL4A1
Specifications
- Immunogen
- Recombinant human Collagen alpha-1(IV) chain, aa 1571-1669, conjugated with KLH.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat COL4A1
- Species Reactivity
- Human, Mouse, Rat, Dog (Predicted), Pig (Predicted), Cow (Predicted), Rabbit (Predicted), Chicken (Predicted), Horse (Predicted)
- Applications
- ELISA, IHC-P, IHC-Fr, FC, IF
- Conjugate
- NONE
- Related Disease
- Retinal Arteries, Tortuosity Of and Schizencephaly
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01× TBS (pH 7.4) containing 50% Glycerol and 1% BSA.
- Preservative
- 0.03% Proclin 300
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- ELISA: 1/5000-1/10000
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Immunohistochemistry-frozen (IHC-Fr): 1/100-1/500
Flow cytometry (FC): 1-2 μg per 1x 10⁶ cells
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- Collagen Type IV Alpha 1 Chain; Collagen Of Basement Membrane, Alpha-1 Chain; Collagen IV, Alpha-1 Polypeptide; Collagen, Type IV, Alpha 1; Collagen Alpha-1(IV) Chain; COL4A1 NC1 Domain;
- Gene ID
- 1282
- UniProt ID
- P02462
- Long Name
- Collagen Type IV Alpha 1 Chain
- Cellular Localization
- Secreted; Extracellular space; Extracellular matrix; Basement membrane
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2110z | Mouse Anti-COL4A1 Recombinant Antibody (clone 35A2) | WB, IHC | Mouse IgG1 |
| VS3-QX263 | Mouse Anti-COL4A1 Recombinant Antibody (clone 2G3-1H1-6E5) | IHC | Mouse IgG1 |
| VS3-QX264 | Mouse Anti-COL4A1 Recombinant Antibody (clone 3E8-2A5-1A10) | IHC | Mouse IgG1 |
| VS3-QX265 | Mouse Anti-COL4A1 Recombinant Antibody (clone 7A10-5B3-7D4) | IHC | Mouse IgG1 |
| VS3-QX266 | Mouse Anti-COL4A1 Recombinant Antibody (clone 7B3-6D3-6A4) | IHC | Mouse IgG1 |
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