Mouse Anti-COL4A1 Recombinant Antibody (clone 8E5)

Name: Mouse Anti-COL4A1 Recombinant Antibody (clone 8E5)
SKU: ZG-0181J
Rating: 5 (1 reviews)

CAT#: ZG-0181J

This product is a mouse antibody that recognizes COL4A1.

Tested Data Gene Expression

Figure 1 Mouse Anti-COL4A1 Recombinant Antibody (clone 8E5) in IF

Immunofluorescence analysis of human-liver tissue. 1.Collagen IV Mouse Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 2 Mouse Anti-COL4A1 Recombinant Antibody (clone 8E5) in IF

Immunofluorescence analysis of rat-lung tissue. 1.Collagen IV Mouse Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

IHC

Figure 3 Mouse Anti-COL4A1 Recombinant Antibody (clone 8E5) in IHC

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1.Collagen IV Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

IHC

Figure 4 Mouse Anti-COL4A1 Recombinant Antibody (clone 8E5) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Collagen IV Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

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Normal Tissue Figure 1 IHC staining of human placenta

Immunohistochemical staining of human placenta shows positivity in basement membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/5012_B_7_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_7_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_4_6.jpg

Normal Tissue Figure 7 Placenta

Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_3_7.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (ASC diff, EFO-21, TIME)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187498-COL4A1

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Specifications

Immunogen
Synthetic Peptide of Collagen IV

Host Species
Mouse

Type
Mouse IgG

Specificity
Human COL4A1

Species Reactivity
Human, Mouse, Rat

Clone
8E5

Applications
IF, ICC, IHC-P

Product Property

Storage
Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin.

Target

Alternative Names
BSVD; BSVD1; RATOR; PADMAL; COL4A1s; collagen alpha-1(IV) chain; COL4A1 NC1 domain; arresten; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain

Gene ID
1282

UniProt ID
P02462

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0181J.pdf

MSDS

MSDS - ZG-0181J.pdf

COA

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "COL4A1"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
ZG-0178J	Mouse Anti-COL4A1 Recombinant Antibody (clone 4D1)	IHC-P, IF	Mouse IgG
ZG-0179J	Mouse Anti-COL4A1 Recombinant Antibody (clone 5C9)	IHC-P, IF	Mouse IgG
ZG-0180J	Mouse Anti-COL4A1 Recombinant Antibody (clone 5E10)	IHC-P, IF	Mouse IgG
ZG-0182J	Mouse Anti-COL4A1 Recombinant Antibody (clone 8G3)	IHC-P, IF	Mouse IgG
ZG-0183J	Mouse Anti-COL4A1 Recombinant Antibody (clone 9A7)	IHC-P, IF	Mouse IgG

CAT	Product Name	Application	Type
VS13-YC236	CytoStream™ Rabbit Anti-COL4A1 Polyclonal Antibody (VS13-YC236)	ELISA, IHC-P, IHC-Fr, FC, IF	Rabbit IgG

CAT	Product Name	Application
VS-0525-XY1590	Anti-COL4A1 Immunohistochemistry Kit	IHC
VS-0525-XY1591	Anti-Mouse COL4A1 Immunohistochemistry Kit	IHC
VS-0525-XY1592	Anti-Rat COL4A1 Immunohistochemistry Kit	IHC

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