Rabbit Anti-COMT Recombinant Antibody (clone 8E1) (CAT#: ZG-0063U)
The purified Rabbit Anti-COMT Recombinant Antibody (clone 8E1) displayed high specificity against the COMT protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-COMT Antibody (ZG-0063U) in WB
Positive WB detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate. All lanes: COMT Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31, 25 kDa
Observed band size: 31 kDa
IHC
Figure 2 Rabbit Anti-COMT Antibody (ZG-0063U) in IHC
IHC image of ZG-0063U diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human COMT
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human COMT
- Species Reactivity
- Human
- Clone
- 8E1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Schizophrenia and Panic Disorder 1
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-231 | Recombinant Anti-human COMT Antibody | WB, IHC, FuncS | IgG |
| MHH-231 | Recombinant Human Anti-human COMT Antibody | WB, Neut, FuncS | IgG |
| MOB-1690MZ | Recombinant Mouse Anti-Human COMT Antibody (clone 1G4-1A1) | ELISA, WB | Mouse antibody |
| MRO-0379-CN | Rabbit Anti-COMT Recombinant Antibody (clone JG39-48) | WB, IHC | Rabbit IgG |
| VS3-WK535 | Rabbit Anti-COMT Recombinant Antibody (clone JM103-7) | WB, ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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