Rabbit Anti-CYCS Recombinant Antibody (VS13-YC279) (CAT#: VS13-YC279)
The Rabbit Anti-CYCS Recombinant Antibody is specific for human, mouse and rat CYCS. CYCS encodes cytochrome c, involved in apoptosis by facilitating electron transfer and iron ion binding, associated with thrombocytopenia and pathways related to synovial fibroblasts. The anti-CYCS antibody can be used for Western blot (1/1000), Immunocytochemistry (1/50), Immunofluorescence (1/50-1/100), Immunohistochemistry-paraffin (1/50-1/1000).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/if_selected.jpg
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18597/42408_B_6_6_selected.jpg
(Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18597/42408_B_6_6_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12177_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12177_B_8_5.jpg
Figure 4 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_7_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_7_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_7_4.jpg
Figure 6 Kidney
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_9_5.jpg
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_9_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Round or early spermatids
Staining: Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Round or early spermatids
Staining: Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_6_6.jpg
Figure 8 Heart muscle
(Cardiomyocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12177_B_5_6.jpg
(Cardiomyocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12177_B_5_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4222/12109_A_8_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172115-CYCS
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172115-CYCS
Specifications
- Immunogen
- Recombinant human Cytochrome C, aa 20-60.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CYCS
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP
- Conjugate
- NONE
- Related Disease
- Thrombocytopenia and Autosomal Thrombocytopenia With Normal Platelets
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50-1/100
Immunohistochemistry-paraffin (IHC-P): 1/50-1/1000
Target
- Alternative Names
- Cytochrome C, Somatic; CYC; Cytochrome C; THC4; HCS;
- Gene ID
- 54205
- UniProt ID
- P99999
- Long Name
- Cytochrome C, Somatic
- Cellular Localization
- Mitochondrion intermembrane space
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-258 | Recombinant Anti-human CYCS Antibody | ELISA, WB, Neut, FuncS | IgG |
| MHH-258 | Recombinant Human Anti-human CYCS Antibody | WB, IP, FuncS | IgG |
| MRO-0426-CN | Mouse Anti-CYCS Recombinant Antibody (clone 10-E11-G2) | WB, IHC, IF | Mouse IgG1 |
| MRO-0427-CN | Rabbit Anti-CYCS Recombinant Antibody (clone CBACN-171) | WB, IHC, IP | Rabbit IgG |
| ZG-0221J | Mouse Anti-CYCS Recombinant Antibody (clone 4B10) | WB, IF/ICC, IHC-P | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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