Rabbit Anti-CYP19A1 Recombinant Antibody (clone 1H1) (CAT#: ZG-0630J)

Figure 1 Rabbit Anti-CYP19A1 Antibody (ZG-0630J) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate
All lanes: CYP19A1 antibody at 2.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 25 KDa
Observed band size: 58 KDa

Figure 2 Rabbit Anti-CYP19A1 Antibody (ZG-0630J) in IHC

IHC image of this product diluted at 1:230 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-CYP19A1 Antibody (ZG-0630J) in IF

Immunofluorescence staining of HepG2 cells with this product at 1:76, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).