Mouse Anti-DDX3X Recombinant Antibody (ZG-0477F)
CAT#: ZG-0477F
This product is a mouse antibody that recognizes DDX3X.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-DDX3X Recombinant Antibody (ZG-0477F) in WB.
DDX3 mouse mAb (1:1000 dilution) was used to detect DDX3 in Hela, 3T3, C6, COS7, K562, and Jurkat cell lysates by Western blot. Predicted band size: 75KDa. Observed band size: 75KDa.
ICC
Figure 2 Mouse Anti-DDX3X Recombinant Antibody (ZG-0477F) in ICC.
DDX3 mouse mAb (diluted 1:200) was used for immunocytochemical staining of HeLa cells fixed with 4% paraformaldehyde.
IP
Figure 3 Mouse Anti-DDX3X Recombinant Antibody (ZG-0477F) in IP.
Immunoprecipitation analysis of Hela cell lysates using DDX3 mouse mAb.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1648/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000215301-DDX3X
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Specifications
- Immunogen
- Recombinant human DDX3 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human DDX3X
- Species Reactivity
- Human, Rat, Mouse, Monkey
- Applications
- Western Blot, Immunocytochemistry
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Immunocytochemistry: 1:200
Target
- Alternative Names
- DEAD-Box Helicase 3, X-Linked; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked; DEAD (Asp-Glu-Ala-Asp) Box Helicase 3, X-Linked; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 3; DEAD Box Protein 3, X-Chromosomal; Helicase-Like Protein 2; DEAD Box, X Isoform; DDX3; HLP2; ATP dependent RNA helicase DDX3X; ATP-dependent RNA helicase DDX3X; CAP Rf; DBX; DDX14; DDX3X; DDX3X_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked; DEAD box; DEAD box protein 3; DEAD box protein 3 X-chromosomal; DEAD box X isoform; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3; DEAD/H box 3; DEAD/H box 3, X-linked; Fibroblast Growth Factor Inducible 14; Fin14; Helicase like protein 2; Helicase-like protein 2; HLP2; X isoform; X-chromosomal.
- Gene ID
- 1654
- UniProt ID
- O00571
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "DDX3X"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3304z | Mouse Anti-DDX3X Recombinant Antibody (clone 28B7) | WB, IP, IF, IHC, ELISA | Mouse IgG2b, κ |
| MOB-1365MZ | Recombinant Mouse Anti-Human DDX3X Antibody (clone 3364D6b) | WB | Mouse antibody |
| VS3-WK37 | Mouse Anti-DDX3X Recombinant Antibody (clone E3-B9) | WB, ELISA | Mouse IgG2a |
| VS3-XY573 | Mouse Anti-DDX3X Recombinant Antibody (clone 6G8-F4-E3) | WB, ICC | Mouse IgG2a |
| VS3-FY2687 | Mouse Anti-DDX3X Recombinant Antibody (VS3-FY2687) | WB, ICC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY159 | Mouse Anti-NHP DDX3X Recombinant Antibody (clone 3B9E3) | WB, IHC, FC, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1958 | Anti-DDX3X Immunohistochemistry Kit | IHC | |
| VS-0525-XY1959 | Anti-Mouse DDX3X Immunohistochemistry Kit | IHC |
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