Mouse Anti-DDX3X Recombinant Antibody (VS3-FY2687) (CAT#: VS3-FY2687)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• DEAD-box helicase 3, X-linked of human, rat, mouse and monkey origins

• Species Reactivity
• Human, Rat, Mouse, Monkey

• Applications
• WB, ICC, IP

• Conjugate
• Unconjugated, FITC

• Related Disease
• Intellectual Developmental Disorder, X-Linked, Syndromic, Snijders Blok Type and X-Linked Intellectual Disability-Hypotonia-Movement Disorder Syndrome

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunoprecipitation: 1/20
  Western Blot: 1/500-1/1000
  Immunocytochemistry: 1/50-1/200

• Alternative Names
• DEAD-Box Helicase 3, X-Linked; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked; DEAD (Asp-Glu-Ala-Asp) Box Helicase 3, X-Linked; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 3; DEAD Box Protein 3, X-Chromosomal; Helicase-Like Protein 2; DEAD Box, X Isoform; DDX3; HLP2

• Gene ID
• 1654

• UniProt ID
• O00571

• Sequence Similarities
• Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.

• Cellular Localization
• Cell membrane
  Nucleus
  Cytoplasm
  Cytoplasm, Stress granule
  Inflammasome

• Post Translation Modifications
• Phosphorylated by TBK1; the phosphorylation is required for the synergistic induction of IFNB mediated by TBK1 and DDX3X.
  Phosphorylated by IKBKE at Ser-102 after ssRNA viral infection; enhances the induction of INFB promoter by IRF3.
  The cytoplasmic form is highly phosphorylated in the G1/S phase of the cell cycle and much less at G2/M.
  Phosphorylation by CSNK1E may inhibit RNA-stimulated ATPase activity.
  Upon stimulation of death receptors, including TNFRSF10B, recruited to receptors and cleaved by caspases.
  Proteolytic fragments remain associated with the receptors.
  This cleavage presumably inactivates DDX3X anti-apoptotic function.
  Ubiquitination at Lys55 and Lys554
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyGen 7 sites, 1 O-linked glycan (7 sites)

• Protein Refseq
• XP_011542194.1; XP_054182567.1; NP_001180345.1; NP_001180346.1; NP_001347.3

• Function
• Multifunctional ATP-dependent RNA helicase (PubMed:17357160, 21589879, 31575075).
  The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity (PubMed:29222110).
  In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs (PubMed:17357160, 21589879).
  Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA (PubMed:30256975).
  Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities (Probable).
  Involved in transcription regulation.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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