Mouse Anti-NHP DDX3X Recombinant Antibody (clone 3B9E3)
CAT#: VS-1024-XY159
The mouse-derived monoclonal antibody targets DDX3X with reactivity against human, mouse, non-human primate, and rat specimens. The immunogen comprises a purified recombinant fragment of human DDX3X, ensuring exceptional specificity. Suitable for Western blot (WB), immunohistochemistry (IHC), flow cytometry (FC), and ELISA, this antibody provides comprehensive utility across multiple experimental platforms. Its broad application spectrum and high sensitivity make it an invaluable tool for in-depth studies of DDX3X expression and function.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1648/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000215301-DDX3X
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Specifications
- Immunogen
- Purified recombinant fragment of human DDX3X
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- DDX3X
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 3B9E3
- Applications
- WB, IHC, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
- Alternative Names
- DEAD-Box Helicase 3; X-Linked; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3; X-Linked; DEAD (Asp-Glu-Ala-Asp) Box Helicase 3; X-Linked; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 3; DEAD Box Protein 3; X-Chromosomal; Helicase-Like Protein 2; DEAD Box; X Isoform; DDX3; HLP2
- Gene ID
- 1654
- UniProt ID
- O00571
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DDX3X"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3304z | Mouse Anti-DDX3X Recombinant Antibody (clone 28B7) | WB, IP, IF, IHC, ELISA | Mouse IgG2b, κ |
| MOB-1365MZ | Recombinant Mouse Anti-Human DDX3X Antibody (clone 3364D6b) | WB | Mouse antibody |
| ZG-0477F | Mouse Anti-DDX3X Recombinant Antibody (ZG-0477F) | WB, ICC | Mouse IgG |
| VS3-WK37 | Mouse Anti-DDX3X Recombinant Antibody (clone E3-B9) | WB, ELISA | Mouse IgG2a |
| VS3-FY2687 | Mouse Anti-DDX3X Recombinant Antibody (VS3-FY2687) | WB, ICC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1958 | Anti-DDX3X Immunohistochemistry Kit | IHC | |
| VS-0525-XY1959 | Anti-Mouse DDX3X Immunohistochemistry Kit | IHC |
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