Mouse Anti-DDX3X Recombinant Antibody (clone 28B7)
CAT#: MOB-3304z
This product is a mouse antibody that recognizes human DDX3X. It can be used in the following applications: WB, IP, IF, IHC, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1648/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_B_1_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59585/159478_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000215301-DDX3X
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Specifications
- Immunogen
- Human DEAD (Asp-Glu-Ala-Asp) Box Helicase 3, X-Linked
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b, κ
- Antibody Isotype
- IgG2b, κ
- Specificity
- Human DDX3X
- Species Reactivity
- Human
- Clone
- 28B7
- Applications
- WB, IP, IF, IHC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunofluorescence Assay, Immunohistochemistry Assay, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- DDX3X; ATP-Dependent RNA Helicase DDX3X; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 3; EC 3.6.4.13; DEAD Box, X Isoform; DDX14; HLP2; EC 3.6.1; DEAD/H Box-3; DEAD (Asp-Glu-Ala-Asp) Box Helicase 3, X-Linked
- UniProt ID
- O00571
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "DDX3X"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1365MZ | Recombinant Mouse Anti-Human DDX3X Antibody (clone 3364D6b) | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1877 | PE-B*39:01/Human DDX3X (SHVAVENAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1880 | PE-B*39:01/Human DDX3X (HVAVENAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1878 | APC-B*39:01/Human DDX3X (SHVAVENAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1881 | APC-B*39:01/Human DDX3X (HVAVENAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1879 | BV421-B*39:01/Human DDX3X (SHVAVENAL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0477F | Mouse Anti-DDX3X Recombinant Antibody (ZG-0477F) | WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK37 | Mouse Anti-DDX3X Recombinant Antibody (clone E3-B9) | WB, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY573 | Mouse Anti-DDX3X Recombinant Antibody (clone 6G8-F4-E3) | WB, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY2687 | Mouse Anti-DDX3X Recombinant Antibody (VS3-FY2687) | WB, ICC, IP | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY159 | Mouse Anti-NHP DDX3X Recombinant Antibody (clone 3B9E3) | WB, IHC, FC, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1958 | Anti-DDX3X Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1959 | Anti-Mouse DDX3X Immunohistochemistry Kit | IHC |
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