CytoStream™ Rabbit Anti-DES Recombinant Antibody (VS13-YC298)
CAT#: VS13-YC298
The CytoStream™ Rabbit Anti-DES Recombinant Antibody is specific for human, mouse and rat DES. DES encodes desmin, an intermediate filament protein crucial for muscle integrity, with mutations associated with muscle dystrophies and cardiomyopathies. The anti-DES antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/1161_A10_3_selected.jpg
Normal Tissue
Figure 2 High expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/43421_B_1_6_val_selected.jpg
Normal Tissue
Figure 3 Low expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/43421_B_7_1_val_selected.jpg
Normal Tissue
Figure 4 High expression in heart muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/34/1988_B_5_6_selected.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/43421_B_1_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HSkMC, LHCN-M2, NB-4, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000175084-DES
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Specifications
- Immunogen
- Recombinant human Desmin, aa 421-470.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DES
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Desmin; Intermediate Filament Protein; Cardiomyopathy, Dilated 1F (Autosomal Dominant); Mutant Desmin P.K241E; LGMD1D; LGMD1E;
- Gene ID
- 1674
- UniProt ID
- P17661
- Long Name
- Desmin
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell membrane; Cytoplasm; Intermediate filament; Nucleus
- Protein Refseq
- NP_001918.3
- Function
- Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400).
In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190).
May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DES"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1093MZ | Recombinant Mouse Anti-Human DES Antibody (clone E44) | ICC, IF, IHC-Fr IHC-P, WB | Mouse antibody |
| ZG-0001C | Mouse Anti-DES Recombinant Antibody (clone ABT-DES) | IHC-P, IF | Mouse IgG |
| ZG-0002C | Mouse Anti-DES Recombinant Antibody (clone PT0122) | IHC-P, IF | Mouse IgG |
| ZG-0003C | Mouse Anti-DES Recombinant Antibody (clone 1B12) | IF, ICC, IHC-P | Mouse IgG |
| VS7-HM813 | Mouse Anti-DES Recombinant Antibody (clone CBL195HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0971 | Hi-Affi™ Rabbit Anti-DES Recombinant Antibody (clone DS971AB) | WB, IHC-P | Rabbit IgG |
| MOR-0087-FY | Rabbit Anti-DES Recombinant Antibody (clone AFY0058) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX325 | Mouse Anti-DES Recombinant Antibody (clone 290CT7.1.1) | WB | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY161 | Mouse Anti-NHP DES Recombinant Antibody (clone D33) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1976 | Anti-DES Immunohistochemistry Kit | IHC | |
| VS-0525-XY1977 | Anti-Mouse DES Immunohistochemistry Kit | IHC | |
| VS-0525-XY1978 | Anti-Rat DES Immunohistochemistry Kit | IHC |
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