Rabbit Anti-DLG4 Recombinant Antibody (clone 1H4)
CAT#: ZG-0090U
The purified Rabbit Anti-DLG4 Recombinant Antibody (clone 1H4) displayed high specificity against the DLG4 protein of human, mouse and rat.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-DLG4 Antibody (ZG-0090U) in WB
Positive WB detected in: Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: PSD95 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 81, 86, 81 kDa
Observed band size: 95 kDa
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Normal Tissue
Figure 1 High expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10122/24907_B_7_5_val_selected.jpg
Normal Tissue
Figure 2 Low expression in liver
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10122/24907_A_8_4_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71006/170683_B_7_5_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1999/5316_B_8_5.jpg
Normal Tissue
Figure 5 Cerebellum
Bergmann glia - cytoplasm/membrane Staining: Not detected Intensity: Negative Bergmann glia - nucleus Staining: Not detected Intensity: Negative GLUC cells - cytoplasm/membrane Staining: Medium Intensity: Moderate GLUC cells - nucleus Staining: Not detected Intensity: Negative Granular cells - cytoplasm/membrane Staining: Not detected Intensity: Negative Granular cells - nucleus Staining: Medium Intensity: Moderate Molecular layer - neuropil Staining: Not detected Intensity: Negative Molecular layer cells - cytoplasm/membrane Staining: High Intensity: Strong Molecular layer cells - nucleus Staining: Not detected Intensity: Negative Processes in granular layer Staining: Not detected Intensity: Negative Processes in molecular layer Staining: Not detected Intensity: Negative Processes in white matter Staining: Not detected Intensity: Negative Purkinje cells - cytoplasm/membrane Staining: Medium Intensity: Moderate Purkinje cells - dendrites Staining: Not detected Intensity: Negative Purkinje cells - nucleus Staining: Not detected Intensity: Negative Synaptic glomeruli - capsule Staining: Not detected Intensity: Negative Synaptic glomeruli - core Staining: Medium Intensity: Moderate White matter cells - cytoplasm/membrane Staining: Not detected Intensity: Negative White matter cells - nucleus Staining: Not detected Intensity: Negative
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1999/5316_B_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132535-DLG4
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Specifications
- Immunogen
- A synthesized peptide derived from human PSD95
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DLG4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 1H4
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Intellectual Developmental Disorder, Autosomal Dominant 62 and Encephalopathy, Progressive, Early-Onset, With Episodic Rhabdomyolysis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| MOB-2430CT | Recombinant Mouse anti-Human DLG4 Monoclonal antibody (nBcdbn66270) | WB, IP, IHC-P, IHC-Fr, IEM | |
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| VS-0522-LC14 | Rat Anti-DLG4 Recombinant Antibody (VS-0522-LC14) | IB, IP, WB, IF | Rat IgG2b |
| VS-0522-LC15 | Human Anti-DLG4 Recombinant Antibody (VS-0522-LC15) | IB, IP, WB, IF | Human IgG1 |
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| CAT | Product Name | Application | Type |
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| NEUT-704CQ | Mouse Anti-DLG4 Recombinant Antibody (clone 6G6-1C9) | Block, ELISA, FC, ICC, IF, IHC, IP, WB, ChIP | Mouse IgG2a |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0997 | Hi-Affi™ Rabbit Anti-DLG4 Recombinant Antibody (clone DS997AB) | WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| VS13-YC306 | CytoStream™ Rabbit Anti-DLG4 Recombinant Antibody (VS13-YC306) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2011 | Anti-DLG4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2012 | Anti-Mouse DLG4 Immunohistochemistry Kit | IHC |
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