Anti-DLG4 Intrabody [Tat] (IAB-B004(T))
Recombinant anti-DLG4 intrabody in fusion with HIV Tat works within cells directed against endogenous neuronal protein DLG4, expressed in E. coli cells. The intrabody can report the localization and amount of endogenous synaptic proteins in living neurons and thus may be used to study changes in synaptic strength in vivo.
Specifications
- Immunogen
- human discs, large homolog 4 (Drosophila)
- Host
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Immunofluorescence; Flow Cytometry; Functional studies
Target
- Alternative Names
- DLG4; discs, large homolog 4 (Drosophila); PSD95; SAP90; SAP-90; disks large homolog 4; discs large homolog 4; Tax interaction protein 15
- Entrez Gene ID
- 1742
- UniProt ID
- P78352
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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