Recombinant Anti-human DLG4 Intrabody [Tat]
CAT#: IAB-B004(T)
Recombinant anti-DLG4 intrabody in fusion with HIV Tat works within cells directed against endogenous neuronal protein DLG4, expressed in E. coli cells. The intrabody can report the localization and amount of endogenous synaptic proteins in living neurons and thus may be used to study changes in synaptic strength in vivo.
Gene Expression
Normal Tissue
Figure 1 High expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10122/24907_B_7_5_val_selected.jpg
Normal Tissue
Figure 2 Low expression in liver
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10122/24907_A_8_4_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71006/170683_B_7_5_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1999/5316_B_8_5.jpg
Normal Tissue
Figure 5 Cerebellum
Bergmann glia - cytoplasm/membrane Staining: Not detected Intensity: Negative Bergmann glia - nucleus Staining: Not detected Intensity: Negative GLUC cells - cytoplasm/membrane Staining: Medium Intensity: Moderate GLUC cells - nucleus Staining: Not detected Intensity: Negative Granular cells - cytoplasm/membrane Staining: Not detected Intensity: Negative Granular cells - nucleus Staining: Medium Intensity: Moderate Molecular layer - neuropil Staining: Not detected Intensity: Negative Molecular layer cells - cytoplasm/membrane Staining: High Intensity: Strong Molecular layer cells - nucleus Staining: Not detected Intensity: Negative Processes in granular layer Staining: Not detected Intensity: Negative Processes in molecular layer Staining: Not detected Intensity: Negative Processes in white matter Staining: Not detected Intensity: Negative Purkinje cells - cytoplasm/membrane Staining: Medium Intensity: Moderate Purkinje cells - dendrites Staining: Not detected Intensity: Negative Purkinje cells - nucleus Staining: Not detected Intensity: Negative Synaptic glomeruli - capsule Staining: Not detected Intensity: Negative Synaptic glomeruli - core Staining: Medium Intensity: Moderate White matter cells - cytoplasm/membrane Staining: Not detected Intensity: Negative White matter cells - nucleus Staining: Not detected Intensity: Negative
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1999/5316_B_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132535-DLG4
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Specifications
- Immunogen
- human discs, large homolog 4 (Drosophila)
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Immunofluorescence; Flow Cytometry; Functional studies
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B004(A) | Recombinant Anti-human DLG4 Intrabody [(D-Arg)9] | WB, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B004(G) | Recombinant Anti-human DLG4 Intrabody [+36 GFP] | WB, ICC, Neut, FuncS | scFv-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-704CQ | Mouse Anti-DLG4 Recombinant Antibody (clone 6G6-1C9) | Block, ELISA, FC, ICC, IF, IHC, IP, WB, ChIP | Mouse IgG2a |
| NEUT-705CQ | Mouse Anti-DLG4 Recombinant Antibody (clone 7E3-1B8) | Block, FC, ICC, IF, IHC, IHC-Fr, IP, WB, ChIP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0997 | Hi-Affi™ Rabbit Anti-DLG4 Recombinant Antibody (clone DS997AB) | WB | Rabbit IgG |
| MOR-4721 | Hi-Affi™ Rabbit Anti-DLG4 Recombinant Antibody (clone TH235DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1286-CN | Mouse Anti-DLG4 Recombinant Antibody (clone B5-E6) | WB, IF, IHC, FC | Mouse IgG1 |
| MRO-1287-CN | Rabbit Anti-DLG4 Recombinant Antibody (clone CBACN-465) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0090U | Rabbit Anti-DLG4 Recombinant Antibody (clone 1H4) | ELISA, WB | Rabbit IgG |
| VS3-WK814 | Rabbit Anti-DLG4 Recombinant Antibody (VS3-WK814) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS7-HM820 | Mouse Anti-DLG4 Recombinant Antibody (clone 2D5H2) | WB, IHC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC306 | CytoStream™ Rabbit Anti-DLG4 Recombinant Antibody (VS13-YC306) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2011 | Anti-DLG4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2012 | Anti-Mouse DLG4 Immunohistochemistry Kit | IHC |
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