Rabbit Anti-DNMT3A Recombinant Antibody (VS13-YC316) (CAT#: VS13-YC316)
The Rabbit Anti-DNMT3A Recombinant Antibody is specific for human and mouse DNMT3A. DNMT3A, involved in de novo methylation, impacts X-chromosome inactivation and development, with key roles in imprinting and cellular epigenetic transitions. The anti-DNMT3A antibody can be used for Western blot (1/1000-1/5000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200).
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/607_H4_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/607_H4_2_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_8_5_selected.jpg
(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_8_5_selected.jpg
Figure 3 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9469/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9469/ihc_selected.jpg
Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/609_H4_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/609_H4_1_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/604_H4_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/604_H4_1_red_green.jpg
Figure 6 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_B_8_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_B_8_5.jpg
Figure 7 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_8_3.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_8_3.jpg
Figure 8 Liver
(Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_8_4.jpg
(Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_8_4.jpg
Figure 9 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_7_5.jpg
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_7_5.jpg
Figure 10 Testis
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_6_6.jpg
(Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_6_6.jpg
Figure 11 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_9_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26588/55045_A_9_8.jpg
Figure 12 RNA cell line category: Cell line enhanced (NTERA-2, REH, SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119772-DNMT3A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119772-DNMT3A
Specifications
- Immunogen
- Recombinant human Dnmt3a, aa 600-640.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse DNMT3A
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF
- Conjugate
- NONE
- Related Disease
- Tatton-Brown-Rahman Syndrome; Heyn-Sproul-Jackson Syndrome
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- DNA Methyltransferase 3 Alpha; DNA (Cytosine-5-)-Methyltransferase 3 Alpha; DNA MTase HsaIIIA; EC 2.1.1.37; M.HsaIIIA; DNA (Cytosine-5)-Methyltransferase 3A;
- Gene ID
- 1788
- UniProt ID
- Q9Y6K1
- Long Name
- DNA Methyltransferase 3 Alpha
- Cellular Localization
- Nucleus; Chromosome; Cytoplasm
- Protein Refseq
- XP_005264232.1; XP_011530964.1
- Function
- It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-311 | Human Anti-DNMT3A Recombinant Antibody (clone D422) | ELISA, IP-MS | Human IgG |
| VS3-WK86 | Mouse Anti-DNMT3A Recombinant Antibody (clone F5-G11) | WB, ELISA | Mouse IgG2a |
| VS3-WK1235 | Rabbit Anti-DNMT3A Recombinant Antibody (VS3-WK1235) | WB, ICC, IF | Rabbit IgG |
| VS3-XY3335 | Rabbit Anti-DNMT3A Recombinant Antibody (clone R02-3C2) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS3-FY2691 | Mouse Anti-DNMT3A Recombinant Antibody (VS3-FY2691) | WB | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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