Mouse Anti-EDAR Recombinant Antibody (clone EDAR9) (CAT#: FAMAB-0090JF)
Recombinant mouse monoclonal antibody specially binds to ectodysplasin A receptor. This anti-EDAR antibody (EDAR9)can be potiently used in the treatment of EDAR-related diseases.
Specific Inquiry
ELISA
Figure 1 Epitope mapping of anti-EDAR monoclonal antibodies.
The indicated EDAR-Fc constructs or B cell maturation antigen-Fc (BCMA-Fc) control were captured in an ELISA plate and revealed with the indicated anti-EDAR antibodies, with an anti-human IgG to control efficient capture of the various EDAR-Fc proteins, or with FLAG-EDA1 or FLAG-BAFF as controls.
Kowalczyk, C., Dunkel, N., Willen, L., Casal, M. L., Mauldin, E. A., Gaide, O., ... & Schneider, P. (2011). Molecular and therapeutic characterization of anti-ectodysplasin A receptor (EDAR) agonist monoclonal antibodies. Journal of Biological Chemistry, 286(35), 30769-30779.
WB
Figure 2 All antibodies recognized recombinant EDAR by Western blot under nonreducing conditions, but only three reacted with reduced EDAR.
Bovine serum albumin (B; BSA, 2 ug), hEDAR-Fc (E, 20 ng), and hFas-Fc (F, 2 0ng) were resolved by SDS-PAGE under reducing or nonreducing conditions, transferred onto nitrocellulose, and probed with anti-EDAR1-15.
Kowalczyk, C., Dunkel, N., Willen, L., Casal, M. L., Mauldin, E. A., Gaide, O., ... & Schneider, P. (2011). Molecular and therapeutic characterization of anti-ectodysplasin A receptor (EDAR) agonist monoclonal antibodies. Journal of Biological Chemistry, 286(35), 30769-30779.
FC
Figure 3 Cross-species specificity of anti-EDAR antibodies.
Receptors fused to the glycosylphosphatidylinositol anchor of TRAILR3 were expressed in 293 Tcells together with an enhanced GFP tracer (x axis) and stained with Fc-EDA1, Fc-EDA2, or with anti-EDAR antibodies (y axis). Receptor expression wasconfirmed by staining with an antibody directed against the C-terminal portion of TRAIL-R3 present in all fluorescence intensity on a logarithmic scale (100-104).constructs (mAb572).
Kowalczyk, C., Dunkel, N., Willen, L., Casal, M. L., Mauldin, E. A., Gaide, O., ... & Schneider, P. (2011). Molecular and therapeutic characterization of anti-ectodysplasin A receptor (EDAR) agonist monoclonal antibodies. Journal of Biological Chemistry, 286(35), 30769-30779.
Specifications
- Immunogen
- mEDAR-Fc
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human EDAR; mouse EDAR; dog EDAR; rat EDAR; mouse EDAR; chicken EDAR
- Species Reactivity
- Human, Mouse, Canine, Rat, Chicken
- Clone
- EDAR9
- Applications
- WB, FACS, ELISA, FuncS
- Related Disease
- X-linked hypohidrotic ectodermal dysplasia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
- Gene ID
- 10913
- UniProt ID
- Q9UNE0
- Function
- Agonist
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1090 | Hi-Affi™ Rabbit Anti-EDAR Recombinant Antibody (clone DS1090AB) | WB, FC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0045-LS | Human Anti-EDAR Recombinant Antibody (HPAB-0045-LS) | ELISA, WB, FC | Human IgG1 |
| HPAB-0046-LS | Human Anti-EDAR Recombinant Antibody (HPAB-0046-LS) | ELISA, WB, FC | Human IgG2b |
| HPAB-0047-LS | Human Anti-EDAR Recombinant Antibody (HPAB-0047-LS) | ELISA, WB, FC | Human IgG2a |
| HPAB-0048-LS | Human Anti-EDAR Recombinant Antibody (HPAB-0048-LS) | ELISA, WB, FC | Human IgG1 |
| FAMAB-1119CQ | Human Anti-EDAR Recombinant Antibody (clone EDAR3) | Agonist, WB, ELISA | Human IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0045-LS-F(E) | Human Anti-EDAR Recombinant Antibody; Fab Fragment (HPAB-0045-LS-F(E)) | ELISA, WB, FC | Human Fab |
| HPAB-0046-LS-F(E) | Human Anti-EDAR Recombinant Antibody; Fab Fragment (HPAB-0046-LS-F(E)) | ELISA, WB, FC | Human Fab |
| HPAB-0047-LS-F(E) | Human Anti-EDAR Recombinant Antibody; Fab Fragment (HPAB-0047-LS-F(E)) | ELISA, WB, FC | Human Fab |
| FAMAB-0089JF-S(P) | Mouse Anti-EDAR Recombinant Antibody (clone EDAR8); scFv Fragment | WB, FACS, ELISA, FuncS | Mouse scFv |
| FAMAB-0090JF-S(P) | Mouse Anti-EDAR Recombinant Antibody (clone EDAR9); scFv Fragment | WB, FACS, ELISA, FuncS | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0045-LS-S(P) | Human Anti-EDAR Recombinant Antibody; scFv Fragment (HPAB-0045-LS-S(P)) | ELISA, WB, FC | Human scFv |
| HPAB-0046-LS-S(P) | Human Anti-EDAR Recombinant Antibody; scFv Fragment (HPAB-0046-LS-S(P)) | ELISA, WB, FC | Human scFv |
| HPAB-0047-LS-S(P) | Human Anti-EDAR Recombinant Antibody; scFv Fragment (HPAB-0047-LS-S(P)) | ELISA, WB, FC | Human scFv |
| HPAB-0048-LS-S(P) | Human Anti-EDAR Recombinant Antibody; scFv Fragment (HPAB-0048-LS-S(P)) | ELISA, WB, FC | Human scFv |
| FAMAB-0089JF-F(E) | Mouse Anti-EDAR Recombinant Antibody (clone EDAR8); Fab Fragment | WB, FACS, ELISA, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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