Mouse Anti-EIF4E Recombinant Antibody (clone CBL221HM)
CAT#: VS7-HM851
Mouse Anti-EIF4E Recombinant Antibody (clone CBL221HM) is an mouse IgG antibody that recognizes the Human EIF4E by WB, IHC-p, IF, ICC, FCM, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68358/1607_H9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16316/951_E2_2_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_6_6.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151247-EIF4E
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Specifications
- Immunogen
- Antiserum was raised against a synthetic peptide derived from human AMPKα, encompassing the Thr172 phosphorylation site (amino acids 140-189).
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EIF4E
- Species Reactivity
- Human
- Clone
- CBL221HM
- Applications
- WB, IHC-p, IF, ICC, FCM, ELISA
- Conjugate
- None
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity-chromatography
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- PBS
- Preservative
- 50% glycerol, 0.5% BSA, and 0.02% sodium azide.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice pack
Target
- Alternative Names
- EIF4E; EIF-4F 25 KDa Subunit; MRNA Cap-Binding Protein; EIF4E1; EIF4EL1; EIF-4E; AUTS19; EIF4E; Eukaryotic Translation Initiation Factor 4E-Like 1; CBP
- UniProt ID
- P06730
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Related Signaling Pathways
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "EIF4E"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1128 | Hi-Affi™ Rabbit Anti-EIF4E Recombinant Antibody (clone DS1128AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1838-CN | Rabbit Anti-EIF4E Polyclonal Antibody (MRO-1838-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX360 | Mouse Anti-EIF4E Recombinant Antibody (clone 8C8-C10-C12) | WB | Mouse IgG1 |
| VS3-QX361 | Mouse Anti-EIF4E Recombinant Antibody (clone 163CT48.1.9) | WB, ICC | Mouse IgG1, κ |
| VS3-FY489 | Recombinant Rabbit Anti-EIF4E Antibody (clone R04-4H7) | WB, IHC-P, IP | Rabbit IgG |
| VS3-WK2053 | Rabbit Anti-EIF4E (pS209) Recombinant Antibody (clone R77-8A-2) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY752 | Anti-EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2229 | Anti-Mouse EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2228 | Anti-Human EIF4E Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC350 | CytoStream™ Rabbit Anti-EIF4E Recombinant Antibody (VS13-YC350) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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