Rabbit Anti-EPAS1 Recombinant Antibody (clone 6A9)
CAT#: ZG-0651J
This product is a rabbit antibody that recognizes EPAS1.
Tested Data
Gene Expression
IF
Figure 1 Rabbit Anti-EPAS1 Antibody (ZG-0651J) in IF
Immunofluorescence staining of Hela Cells with this product at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31200/1391_B7_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69697/1382_A10_1_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (BEWO, BJ hTERT+, HHSteC, RT4, TIME, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116016-EPAS1
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Specifications
- Immunogen
- A synthesized peptide derived from human HIF-2 alpha
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human EPAS1
- Species Reactivity
- Human
- Clone
- 6A9
- Applications
- ELISA, IF
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunofluorescence.
IF:1:20-1:200
Target
- Alternative Names
- HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2-alpha; HIF2-alpha; PAS domain-containing protein 2; basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73; hypoxia-inducible factor 2 alpha; member of PAS protein 2
- Gene ID
- 2034
- UniProt ID
- Q99814
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EPAS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4055z | Mouse Anti-EPAS1 Recombinant Antibody (clone 13F1) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-0918CT | Recombinant Mouse anti-Human EPAS1 Monoclonal antibody (fq200c) | ELISA, IHC-Fr, IHC-P, WB | |
| MRO-0737-CN | Rabbit Anti-EPAS1 Recombinant Antibody (clone JB24-42) | WB, IHC, FC | Rabbit IgG |
| VS7-0425-WR35 | Mouse Anti-EPAS1 Recombinant Antibody (clone 1H3E12) | IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1161 | Hi-Affi™ Rabbit Anti-EPAS1 Recombinant Antibody (clone DS1161AB) | WB, FC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC359 | CytoStream™ Rabbit Anti-EPAS1 Recombinant Antibody (VS13-YC359) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2303 | Anti-EPAS1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2304 | Anti-Mouse EPAS1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2305 | Anti-Bovine EPAS1 Immunohistochemistry Kit | IHC |
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