Rabbit Anti-EPHB4 Recombinant Antibody (clone R04-3B0) (CAT#: VS3-FY2000)
The R04-3B0 recombinant monoclonal antibody is able to recognize human EPHB4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The ephrin receptor B4 antibody is recommended for detection of target protein by western blot analysis of Eph receptor B4/HTK in MEF lysates.
Specific Inquiry
WB
Figure 1 Anti-EPHB4 Antibody (VS3-FY2000) in WB.
The Eph receptor B4/HTK expression in MEF lysates were detected using the anti-Ephrin Receptor B4 by western blot assay.
Specifications
- Immunogen
- Recombinant human Eph receptor B4/HTK protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EPHB4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3B0
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 108 kDa, while the observed molecular weight stands at 135 kDa.
- Related Disease
- Lymphatic Malformation 7 and Capillary Malformation-Arteriovenous Malformation 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- EPH Receptor B4; Tyrosine-Protein Kinase TYRO11; Hepatoma Transmembrane Kinase; EC 2.7.10.1; TYRO11; MYK1; HTK; Tyrosine-Protein Kinase Receptor HTK; Ephrin Type-B Receptor 4
- Gene ID
- 2050
- UniProt ID
- P54760
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated; autophosphorylation is stimulated by EFNB2. (P54760-EPHB4_HUMAN)
Glycosylation at Asn203, Asn335, and Asn426 (NX_P54760 [NX_P54760-1])
Modification sites at PhosphoSitePlus (P54760)
Glycosylation from GlyConnect EPHB4_HUMAN (1214)
Glycosylation from GlyGen (P54760) 3 sites, 3 N-linked glycans (1 site)
- Protein Refseq
- XP_016867305.1; XP_054213436.1; NP_004435.3
- Function
- Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EPHB4"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Human Antibody Products
Mouse Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
Fab Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2116z | Mouse Anti-EPHB4 Recombinant Antibody (clone 25G10) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| HPAB-0184-CN | Human Anti-EPHB4 Recombinant Antibody (clone 22E6) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0185-CN | Human Anti-EPHB4 Recombinant Antibody (clone 30C2) | ELISA, WB, IP | Humanized IgG1, κ |
| ZG-0303J | Mouse Anti-EPHB4 Recombinant Antibody (ZG-0303J) | WB, ELISA | Mouse IgG |
| VS3-XY638 | Mouse Anti-EPHB4 Recombinant Antibody (clone 7H4A6) | ELISA, WB, IHC, ICC | Mouse IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY2000. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: WB, IF, FuncS
Application: Neut, Inhib, FC, ELISA
-1.jpg)
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
Application: FC
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
