Rabbit Anti-EPHB4 Recombinant Antibody (clone R04-3B0) (CAT#: VS3-FY2000)
The R04-3B0 recombinant monoclonal antibody is able to recognize human EPHB4. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-P. The ephrin receptor B4 antibody is recommended for detection of target protein by western blot analysis of Eph receptor B4/HTK in MEF lysates.
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WB
Figure 1 Anti-EPHB4 Antibody (VS3-FY2000) in WB.
The Eph receptor B4/HTK expression in MEF lysates were detected using the anti-Ephrin Receptor B4 by western blot assay.
Specifications
- Immunogen
- Recombinant human Eph receptor B4/HTK protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EPHB4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3B0
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 108 kDa, while the observed molecular weight stands at 135 kDa.
- Related Disease
- Lymphatic Malformation 7 and Capillary Malformation-Arteriovenous Malformation 2
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- EPH Receptor B4; Tyrosine-Protein Kinase TYRO11; Hepatoma Transmembrane Kinase; EC 2.7.10.1; TYRO11; MYK1; HTK; Tyrosine-Protein Kinase Receptor HTK; Ephrin Type-B Receptor 4
- Gene ID
- 2050
- UniProt ID
- P54760
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated; autophosphorylation is stimulated by EFNB2. (P54760-EPHB4_HUMAN)
Glycosylation at Asn203, Asn335, and Asn426 (NX_P54760 [NX_P54760-1])
Modification sites at PhosphoSitePlus (P54760)
Glycosylation from GlyConnect EPHB4_HUMAN (1214)
Glycosylation from GlyGen (P54760) 3 sites, 3 N-linked glycans (1 site)
- Protein Refseq
- XP_016867305.1; XP_054213436.1; NP_004435.3
- Function
- Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2116z | Mouse Anti-EPHB4 Recombinant Antibody (clone 25G10) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| HPAB-0184-CN | Human Anti-EPHB4 Recombinant Antibody (clone 22E6) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0185-CN | Human Anti-EPHB4 Recombinant Antibody (clone 30C2) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0186-CN | Human Anti-EPHB4 Recombinant Antibody (clone 1E10) | ELISA, WB, IP | Humanized IgG1, κ |
| HPAB-0187-CN | Human Anti-EPHB4 Recombinant Antibody (clone 4F2) | ELISA, WB, IP | Humanized IgG1, κ |
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