Rabbit Anti-EPHB4 Recombinant Antibody (clone R04-3B0) (CAT#: VS3-FY2000)

• Immunogen
• Recombinant human Eph receptor B4/HTK protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat EPHB4

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R04-3B0

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• MW
• The calculated weight of the target protein is approximately 108 kDa, while the observed molecular weight stands at 135 kDa.

• Related Disease
• Lymphatic Malformation 7 and Capillary Malformation-Arteriovenous Malformation 2

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100

• Alternative Names
• EPH Receptor B4; Tyrosine-Protein Kinase TYRO11; Hepatoma Transmembrane Kinase; EC 2.7.10.1; TYRO11; MYK1; HTK; Tyrosine-Protein Kinase Receptor HTK; Ephrin Type-B Receptor 4

• Gene ID
• 2050

• UniProt ID
• P54760

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

• Cellular Localization
• Cell membrane

• Post Translation Modifications
• Phosphorylated; autophosphorylation is stimulated by EFNB2. (P54760-EPHB4_HUMAN)
  Glycosylation at Asn203, Asn335, and Asn426 (NX_P54760 [NX_P54760-1])
  Modification sites at PhosphoSitePlus (P54760)
  Glycosylation from GlyConnect EPHB4_HUMAN (1214)
  Glycosylation from GlyGen (P54760) 3 sites, 3 N-linked glycans (1 site)

• Protein Refseq
• XP_016867305.1; XP_054213436.1; NP_004435.3

• Function
• Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody