Rabbit Anti-ESR1 Recombinant Antibody (clone H17-A), FITC
CAT#: ZG-0425U
Rabbit anti-ESR1 antibody (clone H17-A) was conjugated with FITC under optimal conditions. The FITC-conjugated antibody product displayed high specificity against the ESR1 protein of Human. And the solution is free of unconjugated FITC and unconjugated antibody.
Tested Data
Gene Expression
FC
Figure 1 Rabbit Anti-ESR1 Antibody (ZG-0425U) in FC
Profile of Estrogen Receptor expression in MCF-7 cell line*. MCF-7 cells were fixed, permeabilized and stained with anti-ER FITC (blue, 10 μl per test) or with an isotype control (red).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Endometrium
Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (MCF7, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ESR1
- Species Reactivity
- Human
- Clone
- H17-A
- Applications
- FC
- Conjugate
- FITC
- Related Disease
- Estrogen Resistance and Breast Cancer.
Product Property
- Buffer
- Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C, in the dark. Do not freeze.
Applications
- Application Notes
- This antibody has been used 10 µL per test for Flow Cytometry. Specific conditions should be optimized by the end user.
Target
- Alternative Names
- ER; ESR; Era; ESRA; ESTRR; NR3A1; estrogen receptor; E2 receptor alpha; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha
- Gene ID
- 2099
- UniProt ID
- P03372
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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