AbPlus™ Anti-F11R Magnetic Beads (VS-0724-YC1455)
CAT#: VS-0724-YC1455
The AbPlus Anti-F11R Magnetic Beads (VS-0724-YC1455) is an innovative affinity resin which is bound with anti-F11R specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of F11R protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43616/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43616/1026_E7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43616/965_H10_1_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts
Staining:High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_6_6.jpg
Normal Tissue
Figure 9 Skin
Epidermal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_B_4_8.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61700/138414_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (HaCaT, OE19, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000158769-F11R
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-F11R specific antibody
- Target
- F11R
- Immunogen
- Recombinant human JAM-A extracellular domain, aa 1-242.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
- Alternative Names
- F11 Receptor; Junctional Adhesion Molecule 1; Platelet Adhesion Molecule 1; Platelet F11 Receptor; PAM-1; JAM1; JCAM; Junctional Adhesion Molecule A;
- Gene ID
- 50848
- UniProt ID
- Q9Y624
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| HPAB-0298-CN-F(E) | Human Anti-F11R Recombinant Antibody; Fab Fragment (HPAB-0298-CN-F(E)) | ELISA, WB, IF | Humanized Fab |
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