Recombinant Human Anti-F11R Antibody (Hz6F4-2) (CAT#: HPAB-0137-FY)

Figure 1 Illustrates the antibody binding to JAM-A ECD by ELISA.

Binding activity of chimeric and humanized antibodies (A: heavy chain; B: light chain) to the JAM-A extracellular domain (ECD) was measured by ELISA where an anti-human Ckappa conjugate was used to detect the purified antibodies. Dose-dependent binding activities onto plastic-coated JAM-A ECD were measured at 450 nm

Figure 2 Illustrates the competition binding of biotinylated murine 6F4 to JAM-A ECD by ELISA.

Competition binding of biotinylated murine 6F4 Mab to the JAM-A extracellular domain (ECD) by chimeric and humanized (A: heavy chain; B: light chain) antibodies was measured by ELISA. Dose-dependent displacement of biotinylated 6F4 was monitored onto plastic-coated JAM-A ECD.

Figure 3 Illustrate the Down regulation of JAM-A by the Mab Hz6F4-2 using immunofluorescence staining.

Figure 4 Illustrate the Down regulation of JAM-A by the Mab Hz6F4-2 using A: western-blot, B: densitometry analysis.

Figure 5 Illustrates the effect of the Mab Hz6F4-2 on adhesion molecule expression by western blot.