Mouse Anti-FASLG Recombinant Antibody (clone muF919)
CAT#: VS-0724-YC3
muF919 is an anti-Fas ligand antibody capable of suppressing Fas ligand-induced apoptosis to a high degree. The antibody muF919 was evaluated for its apoptosis-suppression activity, which binds to human Fas ligand with high affinity.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_B_8_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_6_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_B_5_4.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_8_8.jpg
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Specifications
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG
- Specificity
- Human Fas ligand
- Species Reactivity
- Human
- Clone
- muF919
- Applications
- ELISA, WB, FC, Apop
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Flow cytometry: 0.01-50 μg/mL
Target
- Alternative Names
- APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A; APT1LG1; tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas ligand (TNF superfamily, member 6); apoptosis (APO-1) antigen ligand 1; apoptosis antigen ligand; fas antigen ligand; mutant tumor necrosis factor family member 6; tumor necrosis factor ligand 1A
- Gene ID
- 356
- UniProt ID
- P48023
- Long Name
- Fas Ligand
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Cellular Localization
- Cell membrane; Single-pass type II membrane protein
Cytoplasmic vesicle lumen
Lysosome lumen
[FasL intracellular domain]
Nucleus
[Tumor necrosis factor ligand superfamily member 6, soluble form]
Secreted
- Post Translation Modifications
- The soluble form derives from the membrane form by proteolytic processing.
The membrane-bound form undergoes two successive intramembrane proteolytic cleavages.
The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain.
This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD).
FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
N-glycosylated.
Glycosylation enhances apoptotic activity.
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
Glycosylation at Asn184, Asn250, and Asn260.
- Protein Refseq
- NP_000630.1; NP_001289675.1
- Function
- Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells.
Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.
Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses.
TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity).
Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis.
[Tumor necrosis factor ligand superfamily member 6, soluble form]: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways.
Can induce apoptosis but does not appear to be essential for this process.
[FasL intracellular domain]: Cytoplasmic form induces gene transcription inhibition. TNFL6_HUMAN,P48023
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0128-YC-F(E) | Human Anti-FASLG Recombinant Antibody (clone Hu F919); Fab Fragment | ELISA, FC | Humanized Fab |
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