Mouse Anti-FASLG Recombinant Antibody (VS-0724-YC6)
CAT#: VS-0724-YC6
The recombinant antibodies NOK3 to NOK5 specifically react with a Fas ligand present on a cell surface. The isotypes of NOK3, NOK4 and NOK5 are mouse IgM, IgG3 and IgG2a kappa, respectively. NOK3 has cross reacitivity with human and rhesus Fas ligand, but not mouse Fas ligand. NOK3 neutralize the apoptosis induction activity of the soluble Fas ligand against the Fas-expressed cells depending on their concentrations.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_B_8_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_6_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_B_5_4.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_8_8.jpg
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| Sub Cat | Clone | Species Reactivity | Type | Applications | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0724-YC7 | NOK3 | Human, Rhesus | Mouse IgM | ELISA, FC, Inhib | ||||||
| VS-0724-YC8 | NOK4 | Human | Mouse IgG3 | ELISA, FC | ||||||
| VS-0724-YC9 | NOK5 | Human | Mouse IgG2a | ELISA, FC |
More Infomation
Specifications
- Immunogen
- Fas ligand-expressed COS cells
- Host Species
- Mouse
- Derivation
- Hybridoma
- Specificity
- Human Fas ligand
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Flow cytometry: 10 μg/mL
Target
- Alternative Names
- APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A; APT1LG1; tumor necrosis factor ligand superfamily member 6; CD95 ligand; Fas ligand (TNF superfamily, member 6); apoptosis (APO-1) antigen ligand 1; apoptosis antigen ligand; fas antigen ligand; mutant tumor necrosis factor family member 6; tumor necrosis factor ligand 1A
- Gene ID
- 356
- UniProt ID
- P48023
- Long Name
- Fas Ligand
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Cellular Localization
- Cell membrane; Single-pass type II membrane protein
Cytoplasmic vesicle lumen
Lysosome lumen
[FasL intracellular domain]
Nucleus
[Tumor necrosis factor ligand superfamily member 6, soluble form]
Secreted
- Post Translation Modifications
- The soluble form derives from the membrane form by proteolytic processing.
The membrane-bound form undergoes two successive intramembrane proteolytic cleavages.
The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain.
This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD).
FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
N-glycosylated.
Glycosylation enhances apoptotic activity.
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
Glycosylation at Asn184, Asn250, and Asn260.
- Protein Refseq
- NP_000630.1; NP_001289675.1
- Function
- Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells.
Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development.
Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses.
TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity).
Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis.
[Tumor necrosis factor ligand superfamily member 6, soluble form]: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways.
Can induce apoptosis but does not appear to be essential for this process.
[FasL intracellular domain]: Cytoplasmic form induces gene transcription inhibition. TNFL6_HUMAN,P48023
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
| TAB-025WM | Human Anti-FASLG Recombinant Antibody (TAB-025WM) | ELISA | Humanized Antibody |
| TAB-025WM-S(P) | Human Anti-FASLG Recombinant Antibody; scFv Fragment (TAB-025WM-S(P)) | ELISA | Humanized scFv |
| TAB-025WM-F(E) | Human Anti-FASLG Recombinant Antibody; Fab Fragment (TAB-025WM-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-026WM | Rabbit Anti-FASLG Recombinant Antibody (TAB-026WM) | ELISA, IHC | Rabbit IgG |
| TAB-026WM-S(P) | Rabbit Anti-FASLG Recombinant Antibody; scFv Fragment (TAB-026WM-S(P)) | ELISA, IHC | Rabbit scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-027WM | Anti-Human FASLG Recombinant Antibody (E09) | ELISA | Human Antibody |
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| TAB-028WM-S(P) | Anti-Human FASLG Recombinant Antibody scFv Fragment (3E1) | FC | Human Antibody |
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| CAT | Product Name | Application | Type |
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| HPAB-0127-YC-S(P) | Mouse Anti-FASLG Recombinant Antibody (clone mF919); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-0128-YC-S(P) | Human Anti-FASLG Recombinant Antibody (clone Hu F919); scFv Fragment | ELISA, FC | Humanized scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0127-YC-F(E) | Mouse Anti-FASLG Recombinant Antibody (clone mF919); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-0128-YC-F(E) | Human Anti-FASLG Recombinant Antibody (clone Hu F919); Fab Fragment | ELISA, FC | Humanized Fab |
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| HPAB-2825LY-F(E) | Human Anti-FASLG Recombinant Antibody; Fab Fragment (HPAB-2825LY-F(E)) | ELISA | Human Fab |
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