Rabbit Anti-FOXO3 Antibody (VS-0924-YC40) - Cancer Stem Cell Marker
CAT#: VS-0924-YC40
This product is an anti-FOXO3 antibody (VS-0924-YC40), specifically targeting the human FOXO3 protein, a marker for cancer stem cells (CSCs). It can be utilized for detecting FOXO3 in CSC tissues or cells through Immunofluorescence at a concentration of 0.5-2 µg/mL.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63104/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4074/21601_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118689-FOXO3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FOXO3
- Species Reactivity
- Human
- Applications
- IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.05 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- Forkhead Box O3; Forkhead In Rhabdomyosarcoma-Like 1; FKHRL1; FOXO3A; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box Protein O3
- Gene ID
- 2309
- UniProt ID
- O43524
- Long Name
- Forkhead box O3
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
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Related Diseases
Gastric Cancer
Hepatocellular Carcinoma
Related Signaling Pathways
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "FOXO3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1344 | Hi-Affi™ Rabbit Anti-FOXO3 Recombinant Antibody (clone DS1344AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1871-CN | Rabbit Anti-FOXO3 Polyclonal Antibody (MRO-1871-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY560 | Recombinant Rabbit Anti-FOXO3 Antibody (clone R03-2H2) | WB | Rabbit IgG |
| VS3-FY561 | Recombinant Rabbit Anti-FOXO3 (phospho Ser253) Antibody (clone R06-2G6) | WB | Rabbit IgG |
| VS3-XY3361 | Rabbit Anti-FOXO3 Recombinant Antibody (clone R09-3J1) | WB, ICC, IF | Rabbit IgG |
| VS3-WK2258 | Rabbit Anti-FOXO3 (pS253) Recombinant Antibody (VS3-WK2258) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2634 | Anti-FOXO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2635 | Anti-Mouse FOXO3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2636 | Anti-Rat FOXO3 Immunohistochemistry Kit | IHC |
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