Rabbit Anti-FOXO3 Recombinant Antibody (clone 1E1) (CAT#: ZG-0675J)
This product is a rabbit antibody that recognizes FOXO3.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-FOXO3 Antibody (ZG-0675J) in WB
Western Blot
Positive WB detected in Hela whole cell lysate,293 whole cell lysate,A549 whole cell lysate
All lanes Phospho-FOXO3 antibody at 0.96μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 97 KDa
Observed band size: 97 KDa
IHC
Figure 2 Rabbit Anti-FOXO3 Antibody (ZG-0675J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 3 Rabbit Anti-FOXO3 Antibody (ZG-0675J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-FOXO3 (S253)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FOXO3
- Species Reactivity
- Human
- Clone
- 1E1
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Secondary acute leukemia
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; forkhead box protein O3; FOXO3A-; forkhead box O3A; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
- Gene ID
- 2309
- UniProt ID
- O43524
Related Resources
Gastric Cancer
Hepatocellular Carcinoma
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "FOXO3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4019z | Mouse Anti-FOXO3 Recombinant Antibody (clone 9G10) | WB, ELISA, IF, IHC | Mouse IgG2a, κ |
| MRO-0602-CN | Rabbit Anti-FOXO3 Recombinant Antibody (clone SP0054) | WB, IF | Rabbit IgG |
| MRO-1871-CN | Rabbit Anti-FOXO3 Polyclonal Antibody (MRO-1871-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-XY3361 | Rabbit Anti-FOXO3 Recombinant Antibody (clone R09-3J1) | WB, ICC, IF | Rabbit IgG |
| VS3-WK2120 | Rabbit Anti-FOXO3 (pS235) Recombinant Antibody (clone R08-7U1) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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