Rabbit Anti-GDF11 Recombinant Antibody (clone R41-2N-8)

CAT#: VS3-FY2115

Clone R41-2N-8 is a rabbit recombinant antibody that detects human GDF11 protein, a calculated weight of 45 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R41-2N-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).

Gene Expression
Figure 1 IF staining of human cell line U-2 OS Figure 2 IHC staining of human cerebral cortex Figure 3 IF staining of human cell line CACO-2 Figure 4 Cerebral cortex Figure 5 Testis Figure 6 RNA cell line category: Cell line enhanced (HMC-1)

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat GDF11
  • Species Reactivity
  • Human, Rat
  • Clone
  • R41-2N-8
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 45kDa.
  • Related Disease
  • Vertebral Hypersegmentation And Orofacial Anomalies and Orofacial Clefting Syndrome

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Growth Differentiation Factor 11; Bone Morphogenetic Protein 11; BMP-11; GDF-11; BMP11; Growth/Differentiation Factor 11
  • Sequence Similarities
  • Belongs to the TGF-beta family.
  • Cellular Localization
  • Secreted
  • Post Translation Modifications
  • Synthesized as large precursor molecule that undergoes proteolytic cleavage by furin-like proteases (PubMed:31215115).
    This produces an inactive form consisting of the mature C-terminal portion non-covalently bound to its cleaved N-terminal propeptide.
    Activation of the mature form requires additional cleavage of the propeptide by a tolloid-like metalloproteinase.
    Glycosylation at Asn94 (NX_O95390 [NX_O95390-1])
    Modification sites at PhosphoSitePlus (O95390)
    Glycosylation from GlyGen (O95390) 2 sites, 1 O-linked glycan (1 site)
  • Function
  • Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity).
    It is required for proper vertebral patterning and orofacial development.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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