Rabbit Anti-GDF11 Recombinant Antibody (clone R41-2N-8) (CAT#: VS3-FY2115)
Clone R41-2N-8 is a rabbit recombinant antibody that detects human GDF11 protein, a calculated weight of 45 kDa. It cross-reacts with rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R41-2N-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60985/1118_F9_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60985/1118_F9_3_selected.jpg
Figure 3 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60985/1261_D2_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60985/1261_D2_3_blue_red_green.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in a subset of glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69609/161896_B_7_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in a subset of glial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69609/161896_B_7_5_selected.jpg
Figure 4 Cerebral cortex
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69609/161896_B_9_5.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69609/161896_B_9_5.jpg
Figure 5 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69609/161896_A_4_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/69609/161896_A_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135414-GDF11
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135414-GDF11
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat GDF11
- Species Reactivity
- Human, Rat
- Clone
- R41-2N-8
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 45kDa.
- Related Disease
- Vertebral Hypersegmentation And Orofacial Anomalies and Orofacial Clefting Syndrome
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Growth Differentiation Factor 11; Bone Morphogenetic Protein 11; BMP-11; GDF-11; BMP11; Growth/Differentiation Factor 11
- Gene ID
- 10220
- UniProt ID
- O95390
- Sequence Similarities
- Belongs to the TGF-beta family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Synthesized as large precursor molecule that undergoes proteolytic cleavage by furin-like proteases (PubMed:31215115).
This produces an inactive form consisting of the mature C-terminal portion non-covalently bound to its cleaved N-terminal propeptide.
Activation of the mature form requires additional cleavage of the propeptide by a tolloid-like metalloproteinase.
Glycosylation at Asn94 (NX_O95390 [NX_O95390-1])
Modification sites at PhosphoSitePlus (O95390)
Glycosylation from GlyGen (O95390) 2 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006719257.1; XP_054226739.1; NP_005802.1
- Function
- Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity).
It is required for proper vertebral patterning and orofacial development.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-390 | Recombinant Anti-human GDF11 Antibody | ELISA, WB, IP, FuncS | IgG |
| MHH-390 | Recombinant Human Anti-human GDF11 Antibody | ELISA, WB, FC, FuncS | IgG |
| MOB-0265CT | Recombinant Mouse anti-Human GDF11 Monoclonal antibody (EML1029) | IHC-P | |
| MRO-1696-CN | Rabbit Anti-GDF11 Polyclonal Antibody (MRO-1696-CN) | IF, IHC, FC | Rabbit IgG |
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