Mouse Anti-GM2 Recombinant Antibody (clone E1) (CAT#: FAMAB-1396CQ)
This product is a recombinant mouse antibody that recognizes GM2. The antibody was purified by affinity chromatography. It can be used in ELISA, IHC.
Specific Inquiry
ELISA
Figure 1 Specificities of MAbs El (A), A3 (B), and F6 (C) toward different purified glycolipids.
Each well of a 96-well plate was coated with 200 ng of glycolipids in 50 µl of methanol. Reactivities were determined with serial dilutions of the purified antibodies,starting at a concentration of 16 µg/ml.
Alfonso, M., Vázquez, A. M., Carr, A., Haerslev, T., Fernández, L. E., Lanio, M. E., ... & Pérez, R. (1995). T cell-independent B cell response to self-monosialogangliosides: primary response monoclonal antibodies. Hybridoma, 14(3), 209-216.
IHC
Figure 2 Immunohistological staining using the avidin-biotincomplex (ABC) technique and MAb E1.
(A) A non-small-cell lung carcinoma showing both granular cytoplasmtic and distinct membrane positivity; (B) negativity in a metastasis from a colon carcinoma; (C) absence of reactivity in normal lung paren¬chyma. Original magnification: (A) x500; (B and C) x200.
Alfonso, M., Vázquez, A. M., Carr, A., Haerslev, T., Fernández, L. E., Lanio, M. E., ... & Pérez, R. (1995). T cell-independent B cell response to self-monosialogangliosides: primary response monoclonal antibodies. Hybridoma, 14(3), 209-216.
HPTLC-immunostaining
Figure 3 Specificities of MAbs El (A), A3 (B), and F6 (C) toward different purified glycolipids.
Each well of a 96-well plate wascoated with 200 ng of glycolipids in 50 µL of methanol. Reactivities were determined with serial dilutions of the purified antibodies, starting at a concentration of 16 µg/ml.
Alfonso, M., Vázquez, A. M., Carr, A., Haerslev, T., Fernández, L. E., Lanio, M. E., ... & Pérez, R. (1995). T cell-independent B cell response to self-monosialogangliosides: primary response monoclonal antibodies. Hybridoma, 14(3), 209-216.
Specifications
- Immunogen
- GM2 ganglioside incorporated into liposomes
- Host Species
- Mouse
- Type
- Mouse IgG1
- Species Reactivity
- Human
- Clone
- E1
- Applications
- ELISA, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- GM2; GM2 Ganglioside
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-406 | Recombinant Anti-GM2 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-406 | Recombinant Human Anti-GM2 Antibody | WB, Neut, FuncS | IgG |
| HPAB-S0039-YC | Human Anti-GM2 Recombinant Antibody (clone KM8966) | ELISA, ADCC | Human IgG |
| HPAB-S0040-YC | Human Anti-GM2 Recombinant Antibody (clone KM8967) | ELISA, ADCC | Human IgG |
| HPAB-A1367-YJ | Human Anti-GM2 Recombinant Antibody (clone KM966) | ELISA, IF, ADCC, CDC, Cyt, FuncS | Chimeric (mouse/human) IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-406-F(E) | Recombinant Anti-GM2 Antibody Fab Fragment | IP, FuncS | Fab |
| HPAB-S0039-YC-F(E) | Human Anti-GM2 Recombinant Antibody (clone KM8966); Fab Fragment | ELISA, ADCC | Human Fab |
| HPAB-S0040-YC-F(E) | Human Anti-GM2 Recombinant Antibody (clone KM8967); Fab Fragment | ELISA, ADCC | Human Fab |
| FAMAB-0054WJ-F(E) | Human Anti-GM2 Recombinant Antibody (clone L55-81); Fab Fragment | WB | Human Fab |
| MOB-102YJL-F(E) | Mouse Anti-GM2 Recombinant Antibody (clone KM796); Fab Fragment | ELISA, ADCC, CDC, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-406-S(P) | Recombinant Anti-GM2 Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| HPAB-S0039-YC-S(P) | Human Anti-GM2 Recombinant Antibody (clone KM8966); scFv Fragment | ELISA, ADCC | Human scFv |
| HPAB-S0040-YC-S(P) | Human Anti-GM2 Recombinant Antibody (clone KM8967); scFv Fragment | ELISA, ADCC | Human scFv |
| FAMAB-0054WJ-S(P) | Human Anti-GM2 Recombinant Antibody (clone L55-81); scFv Fragment | WB | Human scFv |
| MOB-102YJL-S(P) | Mouse Anti-GM2 Recombinant Antibody (clone KM796); scFv Fragment | ELISA, ADCC, CDC, FuncS | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-320CL | Anti-Human GM2 Recombinant Antibody (BIW-8962) | FC, FuncS, IF | Antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-169CT | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody (DMF10.62.3) | ELISA, FC, IHC | |
| TAB-169CT-S(P) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody scFv Fragment (DMF10.62.3) | ELISA, FC, IHC | |
| TAB-170CT-S(P) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody scFv Fragment (DMF10.167.4) | ELISA, FC, IHC | |
| TAB-169CT-F(E) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody Fab Fragment (DMF10.62.3) | ELISA, FC, IHC | |
| TAB-170CT-F(E) | Anti-Human GM2 (Monosialo-GM2) Recombinant Antibody Fab Fragment (DMF10.167.4) | ELISA, FC, IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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