Human Anti-GM2 Recombinant Antibody (clone L55-81) (CAT#: FAMAB-0054WJ)
This product is a human IgM antibody recognizes melanoma-associated ganglioside GM2.
Specific Inquiry
ELISA
Figure 1 Various glycolipids were tested for the binding activity of L55-81 human MAbin ELISA.
The human MAb (800 ug/ml)was diluted with PBS-4% human serumalbumin: 50; 1:100; 1:200. Glycolipids (3 nmol/well) were coated on eachwell, and all tests were done in duplicate.
Nishinaka, Y., Ravindranath, M. H., & Irie, R. F. (1996). Development of a human monoclonal antibody to ganglioside GM2 with potential for cancer treatment. Cancer research, 56(24), 5666-5671.
Cyt
Figure 2 omplement-dependent cytotoxic activity of L55-8l human MAb to a Gₘ₂-positive human melanoma Ml4 cell line.
ll tests were done in triplicate. The percentageof cytolysis was shown by the percentage of ⁵¹Crrelease calculated according to theformula described in “Materialsand Methods.
Nishinaka, Y., Ravindranath, M. H., & Irie, R. F. (1996). Development of a human monoclonal antibody to ganglioside GM2 with potential for cancer treatment. Cancer research, 56(24), 5666-5671.
IHC
Figure 3 Immunohistochemical staining of M14 cell line.
Twenty pg/mi of biotinylated L55-81 were used for staining.
Nishinaka, Y., Ravindranath, M. H., & Irie, R. F. (1996). Development of a human monoclonal antibody to ganglioside GM2 with potential for cancer treatment. Cancer research, 56(24), 5666-5671.
Specifications
- Host Species
- Human
- Type
- Human IgM
- Specificity
- Human GM2
- Species Reactivity
- Human
- Clone
- L55-81
- Applications
- WB, ELISA, Cyt, IHC
- Related Disease
- Melanoma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The GM2 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Cytotoxicity, Immunohistochemistry.
Target
- Alternative Names
- Ganglioside GM2; GM2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Hexamer Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-406 | Recombinant Anti-GM2 Antibody | ELISA, WB, IF, FuncS | IgG |
| MHH-406 | Recombinant Human Anti-GM2 Antibody | WB, Neut, FuncS | IgG |
| FAMAB-1396CQ | Mouse Anti-GM2 Recombinant Antibody (clone E1) | ELISA, IHC | Mouse IgG1 |
| MOB-102YJL | Mouse Anti-GM2 Recombinant Antibody (clone KM796) | ELISA, ADCC, CDC, FuncS | Mouse IgG1 |
| HPAB-A1367-YJ | Human Anti-GM2 Recombinant Antibody (clone KM966) | ELISA, IF, ADCC, CDC, Cyt, FuncS | Chimeric (mouse/human) IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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