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    Rat Anti-GPRC5D Recombinant Antibody (clone 2A4) (CAT#: VS-0824-CJ1)

    Antibody 2A4 is a rat monoclonal antibody targeting the GPRC5D protein. It exhibits species reactivity with both human and cynomolgus monkey, and exhibits ADCC activity against GPRC5D-expressing cells. The affinity of 0.5 nM for antibody 2A4 was determined using SPR. With a high affinity of 1.3 nM assessed by flow cytometry, the epitope of 2A4 is located outside the amino terminus of human GPRC5D.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Normal Tissue
    Figure 1 IHC staining of human pancreas
    Figure 1 IHC staining of human pancreas
    (Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_2_3_selected.jpg
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_B_9_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_7_3.jpg
    Figure 4 Rectum
    Figure 4 Rectum
    (Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_4_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_9_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_4_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/84)
    Figure 9 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/84)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111291-GPRC5D

    Specifications

    • Immunogen
    • Human GPRC5D
    • Host Species
    • Rat
    • Derivation
    • Hybridoma
    • Type
    • Mouse IgG
    • Specificity
    • Human GPRC5D
    • Species Reactivity
    • Human, Monkey
    • Clone
    • 2A4
    • Applications
    • ELISA, FC, FuncS
    • Conjugate
    • Unconjugated
    • Related Disease
    • Myeloma

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • The binding of 2A4 to a human GPRC5D was assessed by ELISA (0.1 ng/mL to 1 µg/mL). The binding of 2A4 to GPRC5D-expressing cells was analyzed by flow cytometry (0.32 ng/mL to 10 µg/mL).

    Target

    • Alternative Names
    • G Protein-Coupled Receptor Class C Group 5 Member D; G Protein-Coupled Receptor, Family C, Group 5, Member D; G Protein-Coupled Receptor, Class C, Group 5, Member D

    Recommended Products

    Isotype Control

    Secondary Antibody

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    See other products for "GPRC5D"

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    CAT Product Name Application Type
    VS-0723-WK42 Human Anti-GPRC5D Recombinant Antibody (clone Talquetamab) FC Human IgG4
    VS-0723-WK191 Rabbit Anti-GPRC5D Recombinant Antibody (VS-0723-WK191) ELISA, FC Rabbit IgG
    VS-0723-WK277 Rabbit Anti-GPRC5D Recombinant Antibody (VS-0723-WK277) ELISA, FC Rabbit IgG
    VS-0723-WK282 Rabbit Anti-GPRC5D Recombinant Antibody (VS-0723-WK282) ELISA, FC Rabbit IgG
    VS-0723-WK288 Rabbit Anti-GPRC5D Recombinant Antibody (VS-0723-WK288) ELISA, FC Rabbit IgG

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