Rat Anti-GPRC5D Recombinant Antibody (clone 2A4)
CAT#: VS-0824-CJ1
Antibody 2A4 is a rat monoclonal antibody targeting the GPRC5D protein. It exhibits species reactivity with both human and cynomolgus monkey, and exhibits ADCC activity against GPRC5D-expressing cells. The affinity of 0.5 nM for antibody 2A4 was determined using SPR. With a high affinity of 1.3 nM assessed by flow cytometry, the epitope of 2A4 is located outside the amino terminus of human GPRC5D.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_2_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_7_3.jpg
Normal Tissue
Figure 4 Rectum
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_4_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71909/157845_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (Karpas-707, RPMI-8226, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111291-GPRC5D
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Specifications
- Immunogen
- Human GPRC5D
- Host Species
- Rat
- Derivation
- Hybridoma
- Type
- Mouse IgG
- Antibody Isotype
- IgG2b
- Specificity
- Human GPRC5D
- Species Reactivity
- Human, Monkey
- Clone
- 2A4
- Applications
- ELISA, FC, FuncS
- Conjugate
- Unconjugated
- Related Disease
- Myeloma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The binding of 2A4 to a human GPRC5D was assessed by ELISA (0.1 ng/mL to 1 µg/mL). The binding of 2A4 to GPRC5D-expressing cells was analyzed by flow cytometry (0.32 ng/mL to 10 µg/mL).
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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