Rabbit Anti-GPX4 Recombinant Antibody (clone R01-6I5)
CAT#: VS3-XY3371
This product is a rabbit recombinant antibody specific for GPX4 (Glutathione peroxidase 4), which plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. It specifically reacts with human, mouse and rat GPX4. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58546/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47224/141864_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47224/141864_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167468-GPX4
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GPX4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-6I5
- Applications
- WB, IHC-F, IHC-P, ICC, IF
- Related Disease
- Male Infertility
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
- Gene ID
- 2879
- UniProt ID
- P36969
- Long Name
- Glutathione peroxidase 4
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys162 and Lys167 (NX_P36969 [NX_P36969-1])
Modification sites at PhosphoSitePlus (P36969)
- Protein Refseq
- NP_001034936.1
- Function
- Required for normal sperm development and male fertility (By similarity).
Essential for maturation and survival of photoreceptor cells (By similarity).
Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity).
Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism (PubMed:11115402).
Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GPX4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0762MZ | Recombinant Mouse Anti-Human GPX4 Antibody (clone 8B5) | ELISA, IP, WB | Mouse antibody |
| MRO-1906-CN | Rabbit Anti-GPX4 Polyclonal Antibody (MRO-1906-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FAMAB-0367JF | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1) | WB, IHC, IF | Mouse IgG2b, κ |
| ZG-0692J | Rabbit Anti-GPX4 Recombinant Antibody (clone 9D4) | ELISA, IHC | Rabbit IgG |
| VS3-FY613 | Recombinant Rabbit Anti-GPX4 Antibody (clone R03-6E6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1499 | Rabbit Anti-GPX4 Recombinant Antibody (clone DS1499AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-F(E) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); Fab Fragment | IB, IP, IHC, ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0367JF-S(P) | Mouse Anti-GPx4 Recombinant Antibody (clone 63-1); scFv Fragment | IB, IP, IHC, ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2900 | Anti-GPX4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2901 | Anti-Mouse GPX4 Immunohistochemistry Kit | IHC |
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