Mouse Anti-GST Recombinant Antibody (clone 3D3E4) (CAT#: ZG-0295U)

Figure 1 Mouse Anti-GST Antibody (ZG-0295U) in WB

Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 26, 102.9, 75.6 KDa
Observed band size: 26, 103, 76 KDa
Exposure time: 1min

Figure 2 Mouse Anti-GST Antibody (ZG-0295U) in IF

Immunofluorescence staining of 293F transfected cells with ZG-0295U at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-GST Antibody (ZG-0295U) in IP

Immunoprecipitating GST in transfected-293F whole cell lysate Lane 1: Mouse control IgG in 293F cell lysate Transfected with GST
Lane 2: ZG-0295U (5µg) + Transfected-293F whole cell lysate (500µg)
Lane 3: Transfected-293F whole cell lysate (10µg)
For western blotting, the blot was detected with ZG-0295U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

Figure 4 Mouse Anti-GST Antibody (ZG-0295U) in FC

Overlay Peak curve showing 293F cells transfected with GST stained with ZG-0295U (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.