Mouse Anti-GST Recombinant Antibody (clone 3D3E4) (CAT#: ZG-0295U)
Mouse anti-GST antibody (clone 3D3E4) displayed high specificity against the GST.
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WB
Figure 1 Mouse Anti-GST Antibody (ZG-0295U) in WB
Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 26, 102.9, 75.6 KDa
Observed band size: 26, 103, 76 KDa
Exposure time: 1min
IF
Figure 2 Mouse Anti-GST Antibody (ZG-0295U) in IF
Immunofluorescence staining of 293F transfected cells with ZG-0295U at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
IP
Figure 3 Mouse Anti-GST Antibody (ZG-0295U) in IP
Immunoprecipitating GST in transfected-293F whole cell lysate Lane 1: Mouse control IgG in 293F cell lysate Transfected with GST
Lane 2: ZG-0295U (5µg) + Transfected-293F whole cell lysate (500µg)
Lane 3: Transfected-293F whole cell lysate (10µg)
For western blotting, the blot was detected with ZG-0295U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
FC
Figure 4 Mouse Anti-GST Antibody (ZG-0295U) in FC
Overlay Peak curve showing 293F cells transfected with GST stained with ZG-0295U (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant GST Protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- GST
- Species Reactivity
- All
- Clone
- 3D3E4
- Applications
- ELISA, WB, IF, FC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:10000-1:640000, IF:1:100-1:300, FC: 1:100-1:300, IP: 1ul-5ul
Target
- Alternative Names
- Glutathione S-Transferase; gst; GST tag
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0323MC | Anti-Glutathione-S-Transferase Antibody | ELISA, WB |
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