AibGenesis™ Rabbit Anti-GTF2F2 Recombinant Antibody (AibG-Rb-GT1495)
CAT#: VS-0326-XY320
Engineered for sensitive detection, this recombinant rabbit antibody targets GTF2F2, a general transcription factor facilitating RNA polymerase II initiation. It is cross-species reactive and supports studies in transcriptional regulation. Dysregulation may influence gene expression.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Round or early spermatids
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6912/18979_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000188342-GTF2F2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- GTF2F2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-GT1495
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Purified
- Format
- Liquid
- Buffer
- PBS with 0.05% protective protein and 50% glycerol.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000-1:2000, Immunohistochemistry: 1:100-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunocytochemistry 1:50-1:200, Immunoprecipitation: 1:20-1:50
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GTF2F2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0242MZ | Chicken Anti-GTF2F2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1541 | Rabbit Anti-GTF2F2 Recombinant Antibody (clone DS1541AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC159 | AbPlus™ Anti-Gtf2f2 Magnetic Beads (VS-0724-YC159) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY974 | Anti-GTF2F2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2981 | Anti-Mouse GTF2F2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2980 | Anti-Human GTF2F2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS14-YC377 | AibGenesis™ Rabbit Anti-GTF2F2 Recombinant Antibody (AibG-Rb-2F177) | WB, IHC, ICC, IF, IP | Rabbit IgG |
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