CytoStream™ Rabbit Anti-HDAC2 Recombinant Antibody (VS13-YC501) (CAT#: VS13-YC501)
The CytoStream™ Rabbit Anti-HDAC2 Recombinant Antibody is specific for human, mouse and rat HDAC2. HDAC2 is involved in transcription regulation and cell cycle progression by forming repressor complexes with various proteins and deacetylating core histone lysine residues. It's an alternative spliced variant of the histone deacetylase family. The anti-HDAC2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11727/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11727/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/if_selected.jpg
Figure 3 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/ihc_selected.jpg
(Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/ihc_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_A_8_8.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_B_7_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_B_7_5.jpg
Figure 6 Salivary gland
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_B_2_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_B_2_1.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_A_9_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_A_9_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196591-HDAC2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196591-HDAC2
Specifications
- Immunogen
- Recombinant human HDAC2, aa 439-488.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HDAC2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Histone Deacetylase 2; EC 3.5.1.98; HD2; Transcriptional Regulator Homolog RPD3; YY1-Associated Factor 1; RPD3; YAF1;
- Gene ID
- 3066
- UniProt ID
- Q92769
- Long Name
- Histone Deacetylase 2
- Cellular Localization
- Nucleus; Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Alcoholism
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3737z | Mouse Anti-HDAC2 Recombinant Antibody (clone 31B12) | WB, IP, IF, IHC | Mouse IgG2b, κ |
| MRO-1931-CN | Rabbit Anti-HDAC2 Polyclonal Antibody (MRO-1931-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS1-YJ82 | Mouse Anti-HDAC2 Recombinant Antibody (clone 3F3) | WB, ICC, IF | Mouse IgG1 |
| VS1-YJ83 | Mouse Anti-HDAC2 Recombinant Antibody (clone 3F3), FITC | WB, ICC, IF | Mouse IgG1 |
| VS1-YJ84 | Mouse Anti-HDAC2 Recombinant Antibody (clone 3F3), HRP | WB, ICC, IF | Mouse IgG1 |
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