CytoStream™ Rabbit Anti-HDAC2 Recombinant Antibody (VS13-YC501)
CAT#: VS13-YC501
The CytoStream™ Rabbit Anti-HDAC2 Recombinant Antibody is specific for human, mouse and rat HDAC2. HDAC2 is involved in transcription regulation and cell cycle progression by forming repressor complexes with various proteins and deacetylating core histone lysine residues. It's an alternative spliced variant of the histone deacetylase family. The anti-HDAC2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11727/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_B_7_5.jpg
Normal Tissue
Figure 6 Salivary gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_B_2_1.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5054/17525_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196591-HDAC2
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Specifications
- Immunogen
- Recombinant human HDAC2, aa 439-488.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HDAC2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
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Related Diseases
Alcoholism
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "HDAC2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2296CT | Recombinant Mouse anti-Human HDAC2 Monoclonal antibody (FQS5045) | IHC-P, WB, IP, ChIP, ICC, IF | |
| MRO-0718-CN | Rabbit Anti-HDAC2 Recombinant Antibody (clone CBACN-257) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-0719-CN | Rabbit Anti-HDAC2 Recombinant Antibody (clone CBACN-258) | WB, IP | Rabbit IgG |
| VS3-FY2733 | Mouse Anti-HDAC2 Recombinant Antibody (VS3-FY2733) | WB, ICC | Mouse IgG |
| VS7-0425-WR20 | Mouse Anti-HDAC2 Recombinant Antibody (clone 4C10C3) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1574 | Rabbit Anti-HDAC2 Recombinant Antibody (clone DS1574AB) | WB, IHC-P, ICC, IF, FC, IP, IHC-Fr | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY120 | AbPlus™ Anti-HDAC2 Magnetic Beads (CBACN-257) | IP, Protein Purification | |
| VS-0724-YC970 | AbPlus™ Anti-HDAC2 Magnetic Beads (VS-0724-YC970) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1000 | Anti-HDAC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3095 | Anti-Mouse HDAC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3094 | Anti-Human HDAC2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3096 | Anti-Rat HDAC2 Immunohistochemistry Kit | IHC |
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