Rabbit Anti-HDAC6 Recombinant Antibody (clone 2H12) (CAT#: ZG-0712J)
This product is a rabbit antibody that recognizes HDAC6.
Specific Inquiry
WB
Figure 1 Rabbit Anti-HDAC6 Antibody (ZG-0712J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: HDAC6 antibody at 1.1μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 132, 115 KDa
Observed band size: 160 KDa
IHC
Figure 2 Rabbit Anti-HDAC6 Antibody (ZG-0712J) in IHC
IHC image of this product diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Figure 3 Rabbit Anti-HDAC6 Antibody (ZG-0712J) in IP
Immunoprecipitating HDAC6 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0712J (3μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human HDAC6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HDAC6
- Species Reactivity
- Human
- Clone
- 2H12
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- HD6; JM21; CPBHM; PPP1R90; histone deacetylase 6; protein phosphatase 1, regulatory subunit 90; tubulin-lysine deacetylase HDAC6
- Gene ID
- 10013
- UniProt ID
- Q9UBN7
Related Resources
Alcoholism
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1577 | Rabbit Anti-HDAC6 Recombinant Antibody (clone DS1577AB) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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