Mouse Anti-NHP HDAC6 Recombinant Antibody (clone OTI4C5)
CAT#: VS-1024-XY241
Designed for comprehensive analysis, this mouse monoclonal antibody targets HDAC6 and exhibits reactivity with dog, human, non-human primate, and rat tissues. The immunogen is a full-length recombinant human HDAC6 protein, ensuring precise antigen recognition. Suitable for Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC), this antibody offers versatile applications for investigating HDAC6 expression and function across a wide range of experimental systems.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26321/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000094631-HDAC6
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Specifications
- Immunogen
- Full length human recombinant protein of HDAC6
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HDAC6
- Species Reactivity
- Dog, Human, Non-human primate, Rat
- Clone
- OTI4C5
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, IHC: 1:200, IF: 1:100, FC: 1:100
Target
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Related Diseases
Alcoholism
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HDAC6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0143CT | Recombinant Mouse anti-Human HDAC6 Monoclonal antibody (EML1253) | ICC, IF, WB | |
| MRO-0722-CN | Rabbit Anti-HDAC6 Recombinant Antibody (clone CBACN-261) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK1186 | Mouse Anti-HDAC6 Recombinant Antibody (VS3-WK1186) | IHC | Mouse IgG1 |
| VS3-WK1187 | Rabbit Anti-HDAC6 Recombinant Antibody (VS3-WK1187) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY3377 | Rabbit Anti-HDAC6 Recombinant Antibody (clone R07-2H6) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1577 | Rabbit Anti-HDAC6 Recombinant Antibody (clone DS1577AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1004 | Anti-HDAC6 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3101 | Anti-Human HDAC6 Immunohistochemistry Kit | IHC |
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