Mouse Anti-NHP HDAC6 Recombinant Antibody (clone OTI4C5) (CAT#: VS-1024-XY241)
Designed for comprehensive analysis, this mouse monoclonal antibody targets HDAC6 and exhibits reactivity with dog, human, non-human primate, and rat tissues. The immunogen is a full-length recombinant human HDAC6 protein, ensuring precise antigen recognition. Suitable for Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry (FC), this antibody offers versatile applications for investigating HDAC6 expression and function across a wide range of experimental systems.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26321/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26321/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_B_7_5.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_7_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_7_5.jpg
Figure 5 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_6_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4236/12458_A_6_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000094631-HDAC6
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000094631-HDAC6
Specifications
- Immunogen
- Full length human recombinant protein of HDAC6
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HDAC6
- Species Reactivity
- Dog, Human, Non-human primate, Rat
- Clone
- OTI4C5
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, IHC: 1:200, IF: 1:100, FC: 1:100
Target
- Alternative Names
- Histone Deacetylase 6; Protein Phosphatase 1; Regulatory Subunit 90; EC 3.5.1.98; HD6; KIAA0901; PPP1R90; CPBHM; JM21;
- Gene ID
- 10013
- UniProt ID
- Q9UBN7
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Alcoholism
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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