Human Anti-HGFAC Recombinant Antibody (clone Ab40) (CAT#: HPAB-S0060-YC)
Ab40 binds to the trypsin like serine protease hepatocyte growth factor activator (HGFA) and has an affinity of 0.2 nM to HGFA. It is a potent inhibitor of HGFA catalytic activity. The antibody binds at the periphery of the substrate binding cleft and imposes a conformational change on the entire 99-loop (chymotrypsinogen numbering).
Specific Inquiry
ELISA
Figure 1 Antibody specificity.
The results demonstrated a complete lack of binding to factor XIIa, urokinase, and matriptase, suggesting good specificity to HGFA.
Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.
Figure 2 Inhibition of HGFA Enzymatic Activity by Ab40.
A. Ab40 inhibited the cleavage of pro-HGF into the α/β-heterodimer mediated by HGFA. B. The enzymatic activity of HGFA was only partially inhibited by Ab40. C. It demonstrated that Ab40 were partial competitive inhibitors of HGFA.
Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.
Figure 3 Effects of Active Site Inhibitors on Antibody Binding to HGFA.
A,B. Surface plasmon resonance studies with HGFA-KQLR complex showed that the irreversibly bound peptidic inhibitor interfered with the binding of Ab40. C. The Kunitz domain inhibitor KD1, which interacts with the extended active-site region, also interferes with Ab40 binding in surface plasmon resonance experiments. D. A competition ELISA showed moderate inhibition of KD1 binding to HGFA by Ab40.
Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.
Specifications
- Immunogen
- HGFA (Val373 - Ser655) was produced by use of a baculovirus and insect cell expression system
- Host Species
- Human
- Type
- Human IgG1
- Clone
- Ab40
- Applications
- ELISA, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- HGF Activator; HGFA; Hepatocyte Growth Factor Activator; EC 3.4.21.-; EC 3.4.21
- Gene ID
- 3083
- UniProt ID
- Q04756
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Human Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463 | Recombinant Anti-human HGFAC Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-463 | Recombinant Human Anti-human HGFAC Antibody | Neut, WB, FuncS | IgG |
| PABL-133 | Recombinant Human Anti-HGFAC Antibody (Ab40) | WB, ELISA, FuncS | IgG |
| PABZ-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab58) | ELISA, Block | Human IgG |
| HPAB-S0059-YC | Human Anti-HGFAC Recombinant Antibody (clone Ab39) | ELISA | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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