Rabbit Anti-Histone H1.3/H1.4 (phospho T17/T17) Recombinant Antibody (VS3-CJ562) (CAT#: VS3-CJ562)

• Immunogen
• Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat phospho-H1.3 (T17)+H1.4 (T17)

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF, IHC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.

• Alternative Names
• H1-3; H1D; H1.3; H1F3; H1s-2; HIST1H1D; H1-4; H1E; H1.4; H1F4; RMNS; H1s-4; HIST1H1E; dJ221C16.5;

• Gene ID
• 3007

• UniProt ID
• P16402

• Sequence Similarities
• Belongs to the histone H1/H5 family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.
  Citrullination at Arg-55 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

• Protein Refseq
• NP_005311.1

• Function
• Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody