Mouse Anti-Histone Recombinant Antibody (clone BWA3) (CAT#: FAMAB-0162YC)
Histones are frequent targets of self-reactive antibodies during autoimmune syndromes. Provided is a mouse anti-histone antibody, named BWA3. BWA3 binds an epitope corresponding to a region of high sequence similarity between H2A and H4 (residues 1-20 and 1-29, respectively).
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-Histone Recombinant Antibody (clone BWA3) (FAMAB-0162YC) in SDS-PAGE
SDS-PAGE analysis of FAMAB-0162YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of FAMAB-0162YC was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Mouse Anti-Histone Recombinant Antibody (clone BWA3) (FAMAB-0162YC) in HPLC
The purity of FAMAB-0162YC was greater than 90% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-Histone Recombinant Antibody (clone BWA3) (FAMAB-0162YC) in WB
Western blot analysis of FAMAB-0162YC was performed by loading recombinant human histone H4 protein.
TAB-388CT incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Mouse Histone
- Species Reactivity
- Mouse
- Clone
- BWA3
- Applications
- IF
- Related Disease
- Autoimmune syndromes
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- H; Histone; Alkaline protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
Fab Fragment Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-465 | Recombinant Anti-Histone Antibody | WB, IF, FuncS | IgG |
| FAMAB-0160YC | Mouse Anti-Histone Recombinant Antibody (clone LG2-1) | IF | Mouse IgG2a, κ |
| FAMAB-0161YC | Mouse Anti-Histone Recombinant Antibody (clone LG2-2) | IF | Mouse IgG2a, κ |
| FAMAB-0175CQ | Mouse Anti-Histone Recombinant Antibody (clone MRL-Histone7) | ELISA | Mouse IgM, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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