Mouse Anti-HK1 Recombinant Antibody (ZG-0474J)
CAT#: ZG-0474J
This product is a mouse antibody that recognizes HK1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HK1 Recombinant Antibody (ZG-0474J) in WB
Western Blot analysis using HXK I Monoclonal Antibody against Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate.
IHC
Figure 2 Mouse Anti-HK1 Recombinant Antibody (ZG-0474J) in IHC
Immunohistochemistry analysis of paraffin-embedded human salivary gland tissues (left) and kidney tissues (right) with DAB staining using HXK I Monoclonal Antibody.
IF
Figure 3 Mouse Anti-HK1 Recombinant Antibody (ZG-0474J) in IF
Immunofluorescence analysis of NIH/3T3 cells using HXK I Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/946_E10_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7043/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7044/ihc_selected.jpg
Normal Tissue
Figure 5 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/ihc_selected.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_6_6.jpg
Normal Tissue
Figure 7 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity: 75%-25% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10052/29589_A_8_4.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156515-HK1
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Specifications
- Immunogen
- Purified recombinant fragment of human HXK I expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HK1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3500z | Mouse Anti-HK1 Recombinant Antibody (clone 18G4) | WB, ELISA, ICC, IF | Mouse IgG2a, κ |
| MOB-0896CT | Recombinant Mouse anti-Human HK1 Monoclonal antibody (5E8) | ELISA, IHC-P, WB | |
| ZG-0419J | Mouse Anti-HK1 Recombinant Antibody (ZG-0419J) | WB | Mouse IgG |
| VS3-QX530 | Mouse Anti-HK1 Recombinant Antibody (clone 6B8-G11-E7) | WB | Mouse IgG1 |
| VS3-FY674 | Recombinant Rabbit Anti-HK1 Antibody (clone R02-4A8) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1614 | Rabbit Anti-HK1 Recombinant Antibody (clone DS1614AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0058-LSX-S(P) | Human Anti-HK1 Recombinant Antibody; scFv Fragment (HPAB-0058-LSX-S(P)) | ELISA | Humanized scFv |
| HPAB-0059-LSX-S(P) | Human Anti-HK1 Recombinant Antibody; scFv Fragment (HPAB-0059-LSX-S(P)) | ELISA | Humanized scFv |
| HPAB-0060-LSX-S(P) | Human Anti-HK1 Recombinant Antibody; scFv Fragment (HPAB-0060-LSX-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0058-LSX-F(E) | Human Anti-HK1 Recombinant Antibody; Fab Fragment (HPAB-0058-LSX-F(E)) | ELISA | Humanized Fab |
| HPAB-0059-LSX-F(E) | Human Anti-HK1 Recombinant Antibody; Fab Fragment (HPAB-0059-LSX-F(E)) | ELISA | Humanized Fab |
| HPAB-0060-LSX-F(E) | Human Anti-HK1 Recombinant Antibody; Fab Fragment (HPAB-0060-LSX-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3159 | Anti-HK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3160 | Anti-Mouse HK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3161 | Anti-Rat HK1 Immunohistochemistry Kit | IHC |
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