Human Anti-HLA-B27 Recombinant Antibody (clone HD5) (CAT#: HPAB-J0121-YC)
This product is a human IgG1 antibody binding to HLA-B27 heavy-chain homodimers, termed HC-B27, HLA-B272 or B272, particularly recognizing B272 homodimers and which does not recognize or bind HLA-B27 heterotrimers (B27) including HLA-B27 heterotrimers with β2 microglobulin and peptide. The antibody is useful in the studies of HLA-B27 mediated conditions, particularly those associated with B272, the spondyloarthritides, a group of related diseases including ankylosing spondylitis (AS) and reactive arthritis (ReA or Reiter's syndrome).
Specific Inquiry
ELISA
Figure 1 HD5 mAb is specific to B272, binds to recombinant B272, and blocks interaction with immunoregulatory cell receptors.
ELISA results showed the specificity of HD5 and HD6 to B272 homodimers when challenged for different recombinant HLA class I complexes (-A1,-B7,-B13,-C7,-B27, and-B272).
Belaunzaran, O. M., Kleber, S., Schauer, S., Hausmann, M., Nicholls, F., Van den Broek, M., ... & Shaw, J. (2015). HLA-B27-homodimer-specific antibody modulates the expansion of pro-inflammatory T-cells in HLA-B27 transgenic rats. Plos one , 10 (6), e0130811.
FC
Figure 2 Representative flow cytometry analysis of HD5 and control antibodies (IgG1 Isotype, W6/32, ME1, HC10, and HD6) binding to LBL721.
HD5 binds specifically to cell-surface B272 homodimers expressed in human 220 B cell lines.
Belaunzaran, O. M., Kleber, S., Schauer, S., Hausmann, M., Nicholls, F., Van den Broek, M., ... & Shaw, J. (2015). HLA-B27-homodimer-specific antibody modulates the expansion of pro-inflammatory T-cells in HLA-B27 transgenic rats. Plos one , 10 (6), e0130811.
Block
Figure 3 HD5 mAb blocks interaction of B272 to leukocytes and modulates activation of CD4+ T-cells by B272.
Pre-incubation of HD5 or HC10 with B272(1x)-tetramers partially blocked interactions with cells when compared to control antibodies.
Belaunzaran, O. M., Kleber, S., Schauer, S., Hausmann, M., Nicholls, F., Van den Broek, M., ... & Shaw, J. (2015). HLA-B27-homodimer-specific antibody modulates the expansion of pro-inflammatory T-cells in HLA-B27 transgenic rats. Plos one , 10 (6), e0130811.
IP
Figure 4 Representative results from 1 of 3 experiments are shown.
Diaminobenzidine‐stained sections of fixed .
Payeli, S. K., Kollnberger, S., Marroquin Belaunzaran, O., Thiel, M., McHugh, K., Giles, J., ... & Keidel, S. (2012). Inhibiting HLA–B27 homodimer–driven immune cell inflammation in spondylarthritis. Arthritis & Rheumatism , 64 (10), 3139-3149.
WB
Figure 5 Western blot analysis of HLA class I heavy chains after surface immunoprecipitation of LBL721.
Immunoprecipitation samples were tested by sodium dodecyl sulfate–polyacrylamide gel electrophoresis under nonreducing conditions (left) and reducing conditions (right).
Payeli, S. K., Kollnberger, S., Marroquin Belaunzaran, O., Thiel, M., McHugh, K., Giles, J., ... & Keidel, S. (2012). Inhibiting HLA–B27 homodimer–driven immune cell inflammation in spondylarthritis. Arthritis & Rheumatism , 64 (10), 3139-3149.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human HLA-B27
- Species Reactivity
- Human
- Clone
- HD5
- Applications
- ELISA, Block, WB, FC, IP
- Related Disease
- Ankylosing spondylitis (AS) and Reactive arthritis (ReA or Reiter's syndrome)
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HLA-B27 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunoprecipitation, Blocking, Flow Cytometry.
Target
- Alternative Names
- Human leukocyte antigen (HLA) B27; B*2701-2759
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody
Recombinant Antibody
scFv Fragment Antibody
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-093CT | Human Anti-HLA-B27 Recombinant Antibody (TAB-093CT) | ELISA, Neut, FC | Human IgG |
| TAB-093CT-S(P) | Human Anti-HLA-B27 Recombinant Antibody; scFv Fragment (TAB-093CT-S(P)) | ELISA, Neut, FC | Human scFv |
| TAB-093CT-F(E) | Human Anti-HLA-B27 Recombinant Antibody; Fab Fragment (TAB-093CT-F(E)) | ELISA, Neut, FC | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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