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    Rabbit Anti-HSF1 (pS326) Recombinant Antibody (clone R77-7H-8) (CAT#: VS3-WK2049)

    This product is a rabbit recombinant antibody that is specific to phosphorylated HSF1. The target of this antibody is phospho-HSF1 (Ser326). The clone R77-7H-8 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, IP analysis at a dilution of 1/20, FC analysis at a dilution of 1/50, and IHC staining at a dilution of 1/100-1/200. The molecular weight of HSF1 (pSer326) observed in WB experiments using this antibody is 82 kDa.

    Sub Cat Clone Applications Application Notes Size Conjugation Endotoxin Purity Fc Engineering  
    VS3-WK2279 R77-7H-8 WB, IHC-P, ICC/IF, IP, FC WB: 1/500-1/1000
    IHC: 1/100-1/200
    IF: 1/50-1/200
    IP: 1/20
    FC: 1/50
    VS3-WK2227 WB, IHC, ICC, IP IP: 1/20
    WB: 1/500-1/1000
    IHC: 1/50-1/100
    ICC: 1/50-1/200
    More Infomation
    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8888/41_C11_2_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_B_8_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_9_3.jpg
    Figure 4 Kidney
    Figure 4 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_7_5.jpg
    Figure 5 Urinary bladder
    Figure 5 Urinary bladder
    (Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_4_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_6_6.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/4239/12078_A_9_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185122-HSF1

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Phospho-Ser326 of human HSF1
    • Species Reactivity
    • Human
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Target

    • Alternative Names
    • Heat Shock Transcription Factor 1; HSTF1; Heat Shock Factor Protein 1; HSTF 1; HSF 1
    • Long Name
    • Heat Shock Transcription Factor 1

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "HSF1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibodies IgG Antibodies NHP Antibodies IHC Kits & Antibodies
    CAT Product Name Application Type
    MOR-1685 Rabbit Anti-HSF1 Recombinant Antibody (clone DS1685AB) ICC, IHC-P, WB Rabbit IgG

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