Mouse Anti-HSPA4 Recombinant Antibody (ZG-0455J)
CAT#: ZG-0455J
This product is a mouse antibody that recognizes HSPA4.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HSPA4 Recombinant Antibody (ZG-0455J) in WB
Western Blot analysis using HSP70 Monoclonal Antibody against HeLa (1) cell lysate.
IHC
Figure 2 Mouse Anti-HSPA4 Recombinant Antibody (ZG-0455J) in IHC
Immunohistochemistry analysis of paraffin-embedded human breast cancer with DAB staining using HSP70 Monoclonal Antibody.
IF
Figure 3 Mouse Anti-HSPA4 Recombinant Antibody (ZG-0455J) in IF
Immunofluorescence analysis of NIH/3T3 cells using HSP70 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/100_C10_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_6_6_selected.jpg
Subcellular Location
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25529/60475_A_1_5_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/101_C10_1_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/82_C10_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/25173_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_7_3.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10023/24928_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170606-HSPA4
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Specifications
- Immunogen
- Purified recombinant fragment of human HSP70 expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPA4
- Species Reactivity
- Human
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-1213z | Mouse Anti-HSPA4 Recombinant Antibody (clone 25G3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| MRO-0787-CN | Rabbit Anti-HSPA4 Recombinant Antibody (clone CBACN-296) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-A1209-YJ | Human Anti-HSPA4 Recombinant Antibody (clone G6A) | ELISA, IF | Human IgG |
| VS3-XY830 | Mouse Anti-HSPA4 Recombinant Antibody (clone 5F2D10) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS7-0425-WR84 | Mouse Anti-HSPA4 Recombinant Antibody (clone 5A6) | WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1694 | Rabbit Anti-HSPA4 Recombinant Antibody (clone DS1694AB) | WB, IP, ICC, ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC698 | AbPlus™ Anti-HSPA4 Magnetic Beads (VS-0724-YC698) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY264 | Mouse Anti-NHP HSPA4 Recombinant Antibody (clone 3A11) | WB | Mouse IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3324 | Anti-HSPA4 Immunohistochemistry Kit | IHC |
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