Mouse Anti-HSPA5 Recombinant Antibody (VS3-FY2739) (CAT#: VS3-FY2739)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Heat shock protein family A (Hsp70) member 5 of human, mouse and rat origins

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IP

• Conjugate
• Unconjugated, FITC

• Related Disease
• Mucormycosis and Japanese Encephalitis

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunoprecipitation: 1/20
  Western Blot: 1/500-1/1000
  Immunocytochemistry: 1/50-1/200

• Alternative Names
• Heat Shock Protein Family A (Hsp70) Member 5; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Immunoglobulin Heavy Chain-Binding Protein; Glucose-Regulated Protein, 78kDa; GRP78; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD)

• Gene ID
• 3309

• UniProt ID
• P11021

• Sequence Similarities
• Belongs to the heat shock protein 70 family.

• Cellular Localization
• Endoplasmic reticulum lumen
  Melanosome
  Cytoplasm
  Cell surface

• Post Translation Modifications
• AMPylated by FICD.
  In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity).
  In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).
  Glycosylation at Thr69, Thr203, and Thr643
  Ubiquitination at Lys96, Lys113, Lys118, Lys213, Lys268, Lys326, Lys370, Lys376, Lys523, Lys547, Lys573, and Lys601
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyGen 6 sites, 2 O-linked glycans (5 sites)

• Protein Refseq
• NP_005338.1

• Function
• Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, 19538957).
  In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity).
  Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).
  Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER).
  May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel.

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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