Mouse Anti-HSPA5 Recombinant Antibody (ZG-0332F)
CAT#: ZG-0332F
This product is a mouse antibody that recognizes HSPA5.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0332F) in WB.
Western blot analysis of extracts from 3T3, A549, C6 and Hela cell lysates was performed using BiP/GRP78 mouse mAb (1:1000 dilution). Predicted band size: 72KDa. Observed band size: 72KDa.
IF
Figure 2 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0332F) in IF.
The BiP/GRP78 mouse mAb (diluted 1:200) was used for immunofluorescence analysis of Hela cells fixed with -20™ anhydrous methanol.
IP
Figure 3 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0332F) in IP.
BiP/GRP78 Mouse mAb was used to immunoprecipitate BiP/GRP78 in Hela cell extracts. Western blot was performed using BiP/GRP78 Mouse mAb.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
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Specifications
- Immunogen
- Recombinant human BiP/GRP78 protein expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPA5
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 5; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Immunoglobulin Heavy Chain-Binding Protein; Glucose-Regulated Protein, 78kDa; GRP78; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); 78 kDa glucose regulated protein; 78 kDa glucose-regulated protein; AL022860; AU019543; BIP; D2Wsu141e; D2Wsu17e; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP-78; GRP78; GRP78_HUMAN; Heat shock 70 kDa protein 5; Heat Shock 70kDa Protein 5; Hsce70; HSPA 5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; Immunoglobulin heavy chain-binding protein; mBiP; MIF2; Sez7.
- Gene ID
- 3309
- UniProt ID
- P11021
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| ZG-0333F | Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) | WB, ICC | Mouse IgG |
| ZG-0448J | Mouse Anti-HSPA5 Recombinant Antibody (ZG-0448J) | WB, IF | Mouse IgG |
| ZG-0449J | Mouse Anti-HSPA5 Recombinant Antibody (ZG-0449J) | WB, IHC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009CT | Mouse Anti-HSPA5 Recombinant Antibody (TAB-009CT) | ELISA, Inhib, FC | Mouse IgG1 |
| TAB-009CT-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (TAB-009CT-S(P)) | ELISA, Inhib, FC | Mouse scFv |
| TAB-009CT-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (TAB-009CT-F(E)) | ELISA, Inhib, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1695 | Rabbit Anti-HSPA5 Recombinant Antibody (clone DS1695AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-035CN | Mouse Anti-HSPA5 Recombinant Antibody (NS-035CN) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-035CN-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (NS-035CN-F(E)) | FC | Mouse Fab |
| NS-035CN-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (NS-035CN-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-S(P) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse scFv |
| HPAB-0369-YJ-S(P) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-F(E) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse Fab |
| HPAB-0369-YJ-F(E) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY234 | Mouse Anti-NHP HSPA5 Recombinant Antibody (clone 6H4-2G7) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY122 | Human Anti-HSPA5 (clone Sam-6) scFv-Fc Chimera | WB, FC, IF, Inhib, ELISA, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3325 | Anti-HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3326 | Anti-Mouse HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3327 | Anti-Rat HSPA5 Immunohistochemistry Kit | IHC |
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