Mouse Anti-HSPD1 Recombinant Antibody (clone 2D3) (CAT#: VS3-WK1611)
This antibody is a mouse recombinant antibody against human HSPD1, which is essential for the folding and assembly of newly imported proteins in the mitochondria. The clone 2D3 can be used in WB analysis at a dilution of 1/1000-1/2000 and IHC analysis at a dilution of 1/50-1/200.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
Figure 3 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
Figure 5 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
Figure 6 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
Figure 7 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
Figure 8 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
Figure 9 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
Figure 10 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
Figure 11 Kidney
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
Figure 12 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
Specifications
- Immunogen
- Synthesized peptide derived from human HSP60
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPD1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 2D3
- Applications
- IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC-P: 1/50-1/200
WB: 1/1000-1/2000
Target
- Alternative Names
- Heat Shock Protein Family D (Hsp60) Member 1; Heat Shock 60kDa Protein 1 (Chaperonin); Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; 0 KDa Chaperonin; Chaperonin 60; HuCHA60; HSP-60; HSP60; CPN60; Spastic Paraplegia 13 (Autosomal Dominant)
- Gene ID
- 3329
- UniProt ID
- P10809
- Long Name
- Heat Shock Protein Family D (Hsp60) Member 1
- Sequence Similarities
- Belongs to the chaperonin (HSP60) family.
- Cellular Localization
- Mitochondrion
- Protein Refseq
- NP_002147.2; NP_955472.1
- Function
- Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Recommended Products
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Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1035 | Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment | ELISA | Mouse Fab |
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| FAMAB-0120JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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