Rabbit Anti-HYOU1 Recombinant Antibody (clone R04-4K7) (CAT#: VS3-FY1810)
Clone R04-4K7 is a rabbit recombinant monoclonal antibody that detects human HYOU1, a 111 kDa member of the immunodlobulin superfamily. It cross-reacts with mouse, rat.
The following dilution ranges are recommended starting points for this product: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/ihc_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_B_8_5.jpg
Figure 3 Colon
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_3.jpg
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_3.jpg
Figure 4 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_2_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_2_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_6_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (EFO-21)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149428-HYOU1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149428-HYOU1
Specifications
- Immunogen
- Synthetic peptide of human ORP150
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HYOU1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4K7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 111 kDa, while the observed molecular weight stands at 150 kDa.
- Related Disease
- Immunodeficiency 59 And Hypoglycemia and B Cell And Dendritic Cell Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Hypoxia Up-Regulated 1; 170 KDa Glucose-Regulated Protein; 150 KDa Oxygen-Regulated Protein; GRP-170; ORP-150; Grp170
- Gene ID
- 10525
- UniProt ID
- Q9Y4L1
- Sequence Similarities
- Belongs to the heat shock protein 70 family.
- Cellular Localization
- Endoplasmic reticulum
- Post Translation Modifications
- Glycosylation at Asn155, Asn222, Asn515, Thr580, Thr589, Thr590, Asn596, Asn830, Asn862, Asn869, Asn922, and Asn931 (NX_Q9Y4L1 [NX_Q9Y4L1-1])
Ubiquitination at Lys398 (NX_Q9Y4L1 [NX_Q9Y4L1-1])
Modification sites at PhosphoSitePlus (Q9Y4L1)
Glycosylation from GlyConnect HYOU1_HUMAN (1383)
Glycosylation from GlyGen (Q9Y4L1) 27 sites, 57 N-linked glycans (8 sites), 4 O-linked glycans (16 sites)
- Protein Refseq
- XP_005271449.1; XP_005271450.1; XP_005271451.1; XP_016872585.1; XP_016872586.1
- Function
- Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1719 | Rabbit Anti-HYOU1 Recombinant Antibody (clone DS1719AB) | WB, IHC, ICC, IP | Rabbit IgG |
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