Rabbit Anti-HYOU1 Recombinant Antibody (clone R04-4K7) (CAT#: VS3-FY1810)
Clone R04-4K7 is a rabbit recombinant monoclonal antibody that detects human HYOU1, a 111 kDa member of the immunodlobulin superfamily. It cross-reacts with mouse, rat.
The following dilution ranges are recommended starting points for this product: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/ihc_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_B_8_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_B_8_5.jpg
Figure 3 Colon
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_3.jpg
(Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_3.jpg
Figure 4 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_2_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_2_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_6_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (EFO-21)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149428-HYOU1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149428-HYOU1
Specifications
- Immunogen
- Synthetic peptide of human ORP150
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HYOU1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4K7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 111 kDa, while the observed molecular weight stands at 150 kDa.
- Related Disease
- Immunodeficiency 59 And Hypoglycemia and B Cell And Dendritic Cell Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Hypoxia Up-Regulated 1; 170 KDa Glucose-Regulated Protein; 150 KDa Oxygen-Regulated Protein; GRP-170; ORP-150; Grp170
- Gene ID
- 10525
- UniProt ID
- Q9Y4L1
- Sequence Similarities
- Belongs to the heat shock protein 70 family.
- Cellular Localization
- Endoplasmic reticulum
- Post Translation Modifications
- Glycosylation at Asn155, Asn222, Asn515, Thr580, Thr589, Thr590, Asn596, Asn830, Asn862, Asn869, Asn922, and Asn931 (NX_Q9Y4L1 [NX_Q9Y4L1-1])
Ubiquitination at Lys398 (NX_Q9Y4L1 [NX_Q9Y4L1-1])
Modification sites at PhosphoSitePlus (Q9Y4L1)
Glycosylation from GlyConnect HYOU1_HUMAN (1383)
Glycosylation from GlyGen (Q9Y4L1) 27 sites, 57 N-linked glycans (8 sites), 4 O-linked glycans (16 sites)
- Protein Refseq
- XP_005271449.1; XP_005271450.1; XP_005271451.1; XP_016872585.1; XP_016872586.1
- Function
- Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1719 | Rabbit Anti-HYOU1 Recombinant Antibody (clone DS1719AB) | WB, IHC, ICC, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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