Rabbit Anti-HYOU1 Recombinant Antibody (clone R04-4K7)
CAT#: VS3-FY1810
Clone R04-4K7 is a rabbit recombinant monoclonal antibody that detects human HYOU1, a 111 kDa member of the immunodlobulin superfamily. It cross-reacts with mouse, rat.
The following dilution ranges are recommended starting points for this product: western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_3.jpg
Normal Tissue
Figure 4 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_2_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49296/109950_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149428-HYOU1
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Specifications
- Immunogen
- Synthetic peptide of human ORP150
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat HYOU1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4K7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 111 kDa, while the observed molecular weight stands at 150 kDa.
- Related Disease
- Immunodeficiency 59 And Hypoglycemia and B Cell And Dendritic Cell Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Hypoxia Up-Regulated 1; 170 KDa Glucose-Regulated Protein; 150 KDa Oxygen-Regulated Protein; GRP-170; ORP-150; Grp170
- Gene ID
- 10525
- UniProt ID
- Q9Y4L1
- Sequence Similarities
- Belongs to the heat shock protein 70 family.
- Cellular Localization
- Endoplasmic reticulum
- Post Translation Modifications
- Glycosylation at Asn155, Asn222, Asn515, Thr580, Thr589, Thr590, Asn596, Asn830, Asn862, Asn869, Asn922, and Asn931 (NX_Q9Y4L1 [NX_Q9Y4L1-1])
Ubiquitination at Lys398 (NX_Q9Y4L1 [NX_Q9Y4L1-1])
Modification sites at PhosphoSitePlus (Q9Y4L1)
Glycosylation from GlyConnect HYOU1_HUMAN (1383)
Glycosylation from GlyGen (Q9Y4L1) 27 sites, 57 N-linked glycans (8 sites), 4 O-linked glycans (16 sites)
- Protein Refseq
- XP_005271449.1; XP_005271450.1; XP_005271451.1; XP_016872585.1; XP_016872586.1
- Function
- Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HYOU1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1719 | Rabbit Anti-HYOU1 Recombinant Antibody (clone DS1719AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0007-DJB | Mouse Anti-HYOU1 Recombinant Antibody (HPAB-0007-DJB) | ELISA, Apop, WB, FC, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0007-DJB-S(P) | Mouse Anti-HYOU1 Recombinant Antibody; scFv Fragment (HPAB-0007-DJB-S(P)) | ELISA, Apop | Mouse scFv |
| HPAB-0735-CN-S(P) | Human Anti-HYOU1 Recombinant Antibody (clone h5F4Ac.2/v17); scFv Fragment | WB, ELISA, IP, FC | Humanized scFv |
| HPAB-AP669-YC-S(P) | Mouse Anti-HYOU1 Recombinant Antibody (clone 3B6.1); scFv Fragment | Apop, CDC, FC | Mouse scFv |
| HPAB-AP670-YC-S(P) | Mouse Anti-HYOU1 Recombinant Antibody (clone 6A4.28); scFv Fragment | Apop, CDC, FC | Mouse scFv |
| HPAB-AP671-YC-S(P) | Mouse Anti-HYOU1 Recombinant Antibody (clone 9A6.2); scFv Fragment | Apop, CDC, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0007-DJB-F(E) | Mouse Anti-HYOU1 Recombinant Antibody; Fab Fragment (HPAB-0007-DJB-F(E)) | ELISA, Apop | Mouse Fab |
| HPAB-AP850-YC-F(E) | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG1); Fab Fragment | Apop, CDC, ELISA, WB | Human Fab |
| HPAB-AP851-YC-F(E) | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG2); Fab Fragment | Apop, CDC, ELISA, WB | Human Fab |
| HPAB-AP852-YC-F(E) | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG3); Fab Fragment | Apop, CDC, ELISA, WB | Human Fab |
| HPAB-AP853-YC-F(E) | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG4); Fab Fragment | Apop, CDC, ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1134-CN | Rabbit Anti-HYOU1 Recombinant Antibody (clone JG39-11) | WB, IHC, FC | Rabbit IgG |
| HPAB-AP850-YC | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG1) | FuncS | Human IgG1 |
| HPAB-AP851-YC | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG2) | FuncS | Human IgG2 |
| HPAB-AP852-YC | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG3) | FuncS | Human IgG3 |
| HPAB-AP853-YC | Human Anti-HYOU1 Recombinant Antibody (clone c5F4-hIgG4) | FuncS | Human IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY138 | AbPlus™ Anti-HYOU1 Magnetic Beads (h5F4Ac.2/v17) | IP, Protein Purification | |
| VS-0724-YC1072 | AbPlus™ Anti-HYOU1 Magnetic Beads (VS-0724-YC1072) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC131 | Mouse Anti-HYOU1 Recombinant Antibody Hexamer (VS-0924-YC131), CDC Enhanced | Apop, CDC, FC | Antibody hexamer |
| VS-0924-YC132 | Human Anti-HYOU1 Recombinant Antibody Hexamer (VS-0924-YC132), CDC Enhanced | CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3364 | Anti-HYOU1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3365 | Anti-Mouse HYOU1 Immunohistochemistry Kit | IHC |
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