Rabbit Anti-IRAK1 Recombinant Antibody (clone R07-6H9) (CAT#: VS3-XY3396)
This antibody is a recombinant rabbit antibody against IRAK1 (Interleukin 1 receptor associated kinase 1). It specifically reacts with human IRAK1. It has been reported for use in Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunoprecipitation and Immunofluorescence is 1/500-1/1000, 1/20 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54476/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54476/if_selected.jpg
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/ihc_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/ihc_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/15546_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/15546_A_7_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/15546_B_7_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/15546_B_7_5.jpg
Figure 5 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/15546_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4461/15546_A_4_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184216-IRAK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184216-IRAK1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IRAK1
- Species Reactivity
- Human
- Clone
- R07-6H9
- Applications
- WB, ICC, IF, IP
- Related Disease
- Pediatric Systemic Lupus Erythematosus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- IRAK; pelle
- Gene ID
- 3654
- UniProt ID
- P51617
- Long Name
- Interleukin 1 receptor associated kinase 1
- Cellular Localization
- Cytoplasm, Nucleus, Lipid droplet
- Post Translation Modifications
- Ubiquitination at Lys134, Lys180, Lys342, Lys355, and Lys397 (NX_P51617 [NX_P51617-1])
Modification sites at PhosphoSitePlus (P51617)
Glycosylation from GlyGen (P51617) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047298054.1
- Function
- Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens.
Involved in Toll-like receptor (TLR) and IL-1R signaling pathways.
Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation.
Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation.
Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
TLR Signaling Pathway
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2474z | Mouse Anti-IRAK1 Recombinant Antibody (clone 23H12) | WB, ELISA, ICC, IF, IHC | Mouse IgG2a |
| MOB-0991CT | Recombinant Mouse anti-Human IRAK1 Monoclonal antibody (EML461) | ICC/IF, IHC-P, WB | |
| VS13-YC594 | Rabbit Anti-IRAK1 Recombinant Antibody (VS13-YC594) | WB, IHC-P | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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