Mouse Anti-ITGA1 Recombinant Antibody (clone 5F9H10)

Name: Mouse Anti-ITGA1 Recombinant Antibody (clone 5F9H10)
SKU: VS7-0425-WR152
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR152

This product is a mouse IgG1 recombinant antibody that binds to human ITGA1. The antibody 5F9H10 recognizes a specific amino acid domain of human ITGA1 (AA: extra(151-364)). It can be used for IHC and FC assays.

Gene Expression

Normal Tissue Figure 1 IHC staining of human colon

Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_B_8_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_7_3.jpg

Normal Tissue Figure 4 Liver

Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_7_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_6_6.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (fHDF/TERT166, HHSteC, SH-SY5Y, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213949-ITGA1

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Specifications

Immunogen
Recombinant human ITGA1 (AA: extra(151-364)) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human ITGA1

Species Reactivity
Human

Clone
5F9H10

Molecule Class
Integrin/Cell Adhesion Molecule

Applications
IHC, FC

Conjugate
Unconjugated

Related Disease
Various forms of collagenopathies; Certain types of cancers; Kidney diseases

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
VLA1; CD49a

Gene ID
3672

UniProt ID
P56199

Long Name
Integrin Subunit Alpha 1

Cellular Localization
Membrane

Post Translation Modifications
Disulfide bond; Glycoprotein

Protein Refseq
NP_852478

Function
Integrin; Receptor

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR152.pdf

MSDS

MSDS - VS7-0425-WR152.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Secondary Antibody

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Product Name

Reconstitution Buffer

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Recommended Dilution Buffer

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