Mouse Anti-ITGA1 Recombinant Antibody (clone 5F9H10)
CAT#: VS7-0425-WR152
This product is a mouse IgG1 recombinant antibody that binds to human ITGA1. The antibody 5F9H10 recognizes a specific amino acid domain of human ITGA1 (AA: extra(151-364)). It can be used for IHC and FC assays.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42555/91585_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (fHDF/TERT166, HHSteC, SH-SY5Y, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213949-ITGA1
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Specifications
- Immunogen
- Recombinant human ITGA1 (AA: extra(151-364)) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ITGA1
- Species Reactivity
- Human
- Clone
- 5F9H10
- Molecule Class
- Integrin/Cell Adhesion Molecule
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Various forms of collagenopathies; Certain types of cancers; Kidney diseases
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| PFBW-108 | Mouse Anti-ITGA1 Recombinant Antibody (clone AQC2); Fab Fragment | ELISA | Mouse Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-108 | Mouse Anti-ITGA1 Recombinant Antibody (clone AQC2); scFv Fragment | ELISA | Mouse scFv |
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| HPAB-1765-FY-F(E) | Human Anti-ITGA1 Recombinant Antibody (clone 2.19); scFv Fragment | ELISA, FuncS | Human scFv |
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|---|---|---|---|
| TAB-356CQ | Human Anti-ITGA1 Recombinant Antibody (TAB-356CQ) | ELISA, FC | Human IgG4, κ |
| TAB-357CQ | Human Anti-ITGA1 Recombinant Antibody (TAB-357CQ) | ELISA, FC | Human IgG4, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| PABX-121-F (E) | Recombinant Mouse Anti-Integrin alpha 1 Antibody Fab Fragment (AQC2) | WB, ELISA, Neut, FuncS | Fab |
| PABX-188-F (E) | Mouse Anti-ITGA1 Recombinant Antibody; Fab Fragment (PABX-188-F (E)) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1541CQ | Recombinant Mouse Anti-ITGA1 Antibody (5E8D9) | Neut, ICC, IF | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3611 | Anti-ITGA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3612 | Anti-Rat ITGA1 Immunohistochemistry Kit | IHC |
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