Mouse Anti-ITGB7 Recombinant Antibody (clone 1B4A8)
CAT#: VS7-0425-WR165
This product is a mouse IgG1 antibody that binds to human ITGB7. The antibody 1B4A8 recognizes specific amino acid domain of human ITGB7 (AA: 20-174). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42277/476_E12_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139626-ITGB7
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Specifications
- Immunogen
- Recombinant human ITGB7 (AA: 20-174) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ITGB7
- Species Reactivity
- Human
- Clone
- 1B4A8
- Molecule Class
- Integrin
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- ITGB7; Gut Homing Receptor Beta Subunit; Integrin, Beta 7
- Gene ID
- 3695
- UniProt ID
- P26010
- Long Name
- Integrin Subunit Beta 7
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_000880.1
- Function
- Host cell receptor for virus entry; Integrin; Receptor
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| MOB-1593z-F(E) | Recombinant Anti-Human ITGB7 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NEUT-1604CQ | Recombinant Rat Anti-Itgb7 Antibody (FIB27) | FC, IP, BL | IgG2a, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1888 | Hi-Affi™ Recombinant Rabbit Anti-ITGB7 Monoclonal Antibody (DS1888AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY135 | Human Anti-ITGB7 scFv-Fc Chimera (VS-0425-FY135) | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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